論文 - 詳細
| RRC ID | 18160 |
|---|---|
| 著者 | Yoshida K, Higuchi C, Nakura A, Yoshikawa H. |
| タイトル | Spleen tyrosine kinase suppresses osteoblastic differentiation through MAPK and PKCα. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
Spleen tyrosine kinase (Syk) is a non-receptor protein kinase present in abundance in a wide range of hematopoietic cells. Syk reportedly plays a crucial role in immune signaling in B cells and cells bearing Fcγ-activation receptors. The role of syk in osteoblastic differentiation has not been well elucidated. We report herein the role of syk in osteoblastic differentiation. We investigated the effects of two syk inhibitors on osteoblastic differentiation in mouse preosteoblastic MC3T3-E1 cells and bone marrow stromal ST2 cells. Expression of syk was detected in these two cell lines. Two syk inhibitors stimulated mRNA expression of osteoblastic markers (ALP, Runx2, Osterix). Mineralization of extracellular matrix was also promoted by treatment with syk inhibitors. Knockdown of Syk caused increased mRNA expression of osteoblastic markers. In addition, syk inhibitor and knockdown of Syk suppressed phosphorylation of mitogen-activated protein kinase (MAPK) and protein kinase Cα (PKCα). Our results indicate that syk might regulate osteoblastic differentiation through MAPK and PKCα. |
| 巻・号 | 411(4) |
| ページ | 774-9 |
| 公開日 | 2011-8-12 |
| DOI | 10.1016/j.bbrc.2011.07.023 |
| PII | S0006-291X(11)01242-3 |
| PMID | 21782794 |
| MeSH | Animals Bone Marrow Cells / cytology Bone Marrow Cells / enzymology Cell Differentiation* Gene Knockdown Techniques Intracellular Signaling Peptides and Proteins / antagonists & inhibitors Intracellular Signaling Peptides and Proteins / genetics Intracellular Signaling Peptides and Proteins / physiology* Mice Mitogen-Activated Protein Kinase Kinases / metabolism* Osteoblasts / cytology* Osteoblasts / enzymology Osteogenesis* Protein Kinase C-alpha / metabolism* Protein Kinase Inhibitors / pharmacology Protein-Tyrosine Kinases / antagonists & inhibitors Protein-Tyrosine Kinases / genetics Protein-Tyrosine Kinases / physiology* Stromal Cells / cytology Stromal Cells / enzymology Syk Kinase |
| IF | 2.985 |
| 引用数 | 5 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | MC3T3-E1(RCB1126) |