論文 - 詳細
| RRC ID | 18185 |
|---|---|
| 著者 | Li Y, Shibata Y, Zhang L, Kuboyama N, Abiko Y. |
| タイトル | Periodontal pathogen Aggregatibacter actinomycetemcomitans LPS induces mitochondria-dependent-apoptosis in human placental trophoblasts. |
| ジャーナル | Placenta |
| Abstract |
OBJECTIVE:Increasing evidence suggests an association between periodontal disease and low birthweight (LBW); however the underlying molecular mechanisms are yet to be fully elucidated. In this study, we performed a microarray analysis to observe the human placental trophoblast-like BeWo cells response to lipopolysaccharide (LPS) from periodontopathogen Aggregatibacter actinomycetemcomitans (Aa), in order to investigate the molecular basis of mechanisms for periodontitis-associated LBW. In vivo pregnant rats were also used to confirm the in vitro results. STUDY DESIGN:The effects of Aa-LPS on cultured human placental trophoblast-like BeWo cells were studied using a DNA microarray, Ingenuity Pathway Analysis, real-time PCR and poly-caspase staining. The in vivo effects of Aa-LPS in pregnant rats were examined using TUNEL assays. RESULTS:In BeWo cells, Aa-LPS increased levels of cytochrome c, caspase 2, caspase 3, caspase 9 and BCL2-antagonist/killer 1 mRNA, decreased those of B-cell CLL/lymphoma 2, BCL2-like 1 and catalase mRNA and increased poly-caspase activity, all of which are consistent with activation of the mitochondria-dependent apoptotic pathway. TUNEL assays confirmed the increased incidence of apoptosis in placentas of Aa-LPS-treated rats (p < 0.001). CONCLUSION:Aa-LPS induces apoptosis in human trophoblasts via the mitochondria-dependent pathway, and this effect may contribute to the pathogenesis of periodontitis-associated LBW. |
| 巻・号 | 32(1) |
| ページ | 11-9 |
| 公開日 | 2011-1-1 |
| DOI | 10.1016/j.placenta.2010.10.007 |
| PII | S0143-4004(10)00385-1 |
| PMID | 21074850 |
| MeSH | Actinobacillus Infections / genetics Actinobacillus Infections / metabolism Actinobacillus Infections / microbiology Actinobacillus Infections / pathology Aggregatibacter actinomycetemcomitans / chemistry* Animals Apoptosis / drug effects* Apoptosis / genetics Cells, Cultured Female Gene Expression Profiling Humans Lipopolysaccharides / isolation & purification Lipopolysaccharides / pharmacology* Microarray Analysis Mitochondria / drug effects Mitochondria / genetics Mitochondria / metabolism Mitochondria / physiology Periodontitis / genetics Periodontitis / metabolism Periodontitis / microbiology* Placenta / cytology Placenta / drug effects Placenta / metabolism Placenta / pathology Pregnancy Pregnancy Complications, Infectious / genetics Pregnancy Complications, Infectious / metabolism Pregnancy Complications, Infectious / microbiology Pregnancy Complications, Infectious / pathology Rats Rats, Sprague-Dawley Trophoblasts / drug effects* Trophoblasts / metabolism Trophoblasts / pathology |
| IF | 3.177 |
| 引用数 | 26 |
| WOS 分野 | OBSTETRICS & GYNECOLOGY REPRODUCTIVE BIOLOGY DEVELOPMENTAL BIOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | BeWo(RCB1644) |