論文 - 詳細
| RRC ID | 18533 |
|---|---|
| 著者 | Cunliffe L, White S, McInerny CJ. |
| タイトル | DSC1-MCB regulation of meiotic transcription in Schizosaccharomyces pombe. |
| ジャーナル | Mol Genet Genomics |
| Abstract |
Meiosis is initiated from the G1 phase of the mitotic cell cycle, and consists of pre-meiotic S-phase followed by two successive nuclear divisions. Here we show that control of gene expression during pre-meiotic S-phase in the fission yeast Schizosaccharomyces pombe is mediated by a DNA synthesis control-like transcription factor complex (DSC1), which acts upon M lu1 cell cycle box (MCB) promoter motifs. Several genes, including rec8+, rec11+, cdc18+, and cdc22+, which contain MCB motifs in their promoter regions, are found to be co-ordinately regulated during pre-meiotic S-phase. Both synthetic and native MCB motifs are shown to confer meiotic-specific transcription on a heterologous reporter gene. A DSC1-like transcription factor complex that binds to MCB motifs was also identified in meiotic cells. The effect of mutating and over-expressing individual components of DSC1 (cdc10+, res1+, res2+, rep1+ and rep2+) on the transcription of cdc22+, rec8+ and rec11+ during meiosis was examined. We found that cdc10+, res2+, rep1+ and rep2+ are required for correct meiotic transcription, while res1+ is not required for this process. This work demonstrates a role for MCB motifs and a DSC1-like transcription factor complex in controlling transcription during meiosis in fission yeast, and suggests a mechanism for how this specific expression occurs. |
| 巻・号 | 271(1) |
| ページ | 60-71 |
| 公開日 | 2004-2-1 |
| DOI | 10.1007/s00438-003-0956-6 |
| PMID | 14648198 |
| MeSH | Base Sequence Cell Cycle Proteins / genetics* Cell Cycle Proteins / metabolism DNA, Fungal / genetics DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism Gene Expression Regulation, Fungal Genes, Fungal Macromolecular Substances Meiosis / genetics* Molecular Sequence Data Mutation S Phase / genetics Schizosaccharomyces / cytology* Schizosaccharomyces / genetics* Schizosaccharomyces / metabolism Schizosaccharomyces pombe Proteins / genetics* Schizosaccharomyces pombe Proteins / metabolism Trans-Activators / genetics Trans-Activators / metabolism Transcription Factors / genetics* Transcription Factors / metabolism Transcription, Genetic |
| IF | 2.797 |
| 引用数 | 13 |
| WOS 分野 | GENETICS & HEREDITY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 酵母 | FYP4207(pREP1) |