論文 - 詳細
| RRC ID | 18705 |
|---|---|
| 著者 | Itoh T, Takeda S, Akao Y. |
| タイトル | MicroRNA-208 modulates BMP-2-stimulated mouse preosteoblast differentiation by directly targeting V-ets erythroblastosis virus E26 oncogene homolog 1. |
| ジャーナル | J Biol Chem |
| Abstract |
MicroRNAs (miRs) represent a class of endogenous approximately 18-25 nucleotide RNAs that regulate gene expression through translational repression by binding to a target mRNA. These miRs regulate several biological functions, such as cell growth, cell differentiation, carcinogenesis, and so on. In a previous report, we have indicated that miR-141 and -200a act as preosteoblast differentiation modulators. In the present study, using microRNA array and in silico analyses, we found that miR-208 is closely involved in preosteoblast differentiation by partially regulating the expression of Ets1 (V-ets erythroblastosis virus E26 oncogene homolog 1), which transactivates osteopontin, runt-related transcription factor 2, parathyroid hormone-related protein, and type I procollagen. Furthermore, the enforced expression of mature miR-208 in murine preosteoblast in MC3T3-E1 cells or primary osteoblast cells remarkably attenuated BMP-2-induced preosteoblast differentiation. In addition, we determined that Ets1 is a target gene of miR-208 by using a sensor luciferase reporter assay. Taken together, these results suggest that the down-regulation of miR-208 in BMP-2-stimulated osteoblast differentiation is an important part of the regulatory machinery involved in early osteogenesis. |
| 巻・号 | 285(36) |
| ページ | 27745-52 |
| 公開日 | 2010-9-3 |
| DOI | 10.1074/jbc.M110.105080 |
| PII | S0021-9258(19)89106-8 |
| PMID | 20576608 |
| PMC | PMC2934642 |
| MeSH | 3T3 Cells Animals Base Sequence Blotting, Western Bone Morphogenetic Protein 2 / pharmacology* Cell Differentiation / drug effects* Cell Differentiation / genetics Computational Biology Enzyme Activation / drug effects Enzyme Activation / genetics Gene Expression Profiling Genes, Reporter / genetics Humans Luciferases / genetics Mice MicroRNAs / genetics* Mitogen-Activated Protein Kinase 1 / metabolism Mitogen-Activated Protein Kinase 3 / metabolism Oligonucleotide Array Sequence Analysis Osteoblasts / cytology* Osteoblasts / drug effects Osteoblasts / enzymology Osteoblasts / metabolism* Osteogenesis / drug effects Osteogenesis / genetics Proto-Oncogene Protein c-ets-1 / deficiency Proto-Oncogene Protein c-ets-1 / genetics* Rats |
| IF | 4.238 |
| 引用数 | 54 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | MC3T3-E1(RCB1126) |