| RRC ID |
19712
|
| 著者 |
Zaratiegui M, Vaughn MW, Irvine DV, Goto D, Watt S, Bähler J, Arcangioli B, Martienssen RA.
|
| タイトル |
CENP-B preserves genome integrity at replication forks paused by retrotransposon LTR.
|
| ジャーナル |
Nature
|
| Abstract |
Centromere-binding protein B (CENP-B) is a widely conserved DNA binding factor associated with heterochromatin and centromeric satellite repeats. In fission yeast, CENP-B homologues have been shown to silence long terminal repeat (LTR) retrotransposons by recruiting histone deacetylases. However, CENP-B factors also have unexplained roles in DNA replication. Here we show that a molecular function of CENP-B is to promote replication-fork progression through the LTR. Mutants have increased genomic instability caused by replication-fork blockage that depends on the DNA binding factor switch-activating protein 1 (Sap1), which is directly recruited by the LTR. The loss of Sap1-dependent barrier activity allows the unhindered progression of the replication fork, but results in rearrangements deleterious to the retrotransposon. We conclude that retrotransposons influence replication polarity through recruitment of Sap1 and transposition near replication-fork blocks, whereas CENP-B counteracts this activity and promotes fork stability. Our results may account for the role of LTR in fragile sites, and for the association of CENP-B with pericentromeric heterochromatin and tandem satellite repeats.
|
| 巻・号 |
469(7328)
|
| ページ |
112-5
|
| 公開日 |
2011-1-6
|
| DOI |
10.1038/nature09608
|
| PII |
nature09608
|
| PMID |
21151105
|
| PMC |
PMC3057531
|
| MeSH |
Centromere Protein B / deficiency
Centromere Protein B / genetics
Centromere Protein B / metabolism*
Chromosomal Proteins, Non-Histone / genetics
Chromosomal Proteins, Non-Histone / metabolism
Conserved Sequence / genetics
DNA Damage / genetics
DNA Replication / genetics*
DNA-Binding Proteins / genetics
DNA-Binding Proteins / metabolism
Genome, Fungal / genetics*
Genomic Instability / genetics*
Recombination, Genetic
Retroelements / genetics*
Schizosaccharomyces / genetics*
Schizosaccharomyces / metabolism
Schizosaccharomyces pombe Proteins / genetics
Schizosaccharomyces pombe Proteins / metabolism
Terminal Repeat Sequences / genetics*
|
| IF |
42.779
|
| 引用数 |
59
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| 酵母 |
FY11012
FY11110 |
