RRC ID 20977
Author Umebayashi H, Mizokami A, Matsuda M, Harada K, Takeuchi H, Tanida I, Hirata M, Kanematsu T.
Title Phospholipase C-related catalytically inactive protein, a novel microtubule-associated protein 1 light chain 3-binding protein, negatively regulates autophagosome formation.
Journal Biochem. Biophys. Res. Commun.
Abstract Upon starvation, cells undergo autophagy, an intracellular bulk-degradation process, to provide the required nutrients. Here, we observed that phospholipase C-related catalytically inactive protein (PRIP) binds to microtubule-associated protein 1 light chain 3 (LC3), a mammalian autophagy-related initiator that regulates the autophagy pathway. Then, we examined the involvement of PRIP in the nutrient depletion-induced autophagy pathway. Enhanced colocalization of PRIP with LC3 was clearly seen in nutrient-starved mouse embryonic fibroblasts under a fluorescent microscope, and interaction of the proteins was revealed by immunoprecipitation experiments with an anti-LC3 antibody. Under starvation conditions, there were more green fluorescent protein fused-LC3 dots in mouse embryonic fibroblasts from PRIP-deficient mice than in fibroblasts from wild type cells. The formation of new dots in a single cell increased, as assessed by time-lapse microscopy. Furthermore, the increase in autophagosome formation in PRIP-deficient cells was notably inhibited by exogenously overexpressed PRIP. Taken together, PRIP is a novel LC3-binding protein that acts as a negative modulator of autophagosome formation.
Volume 432(2)
Pages 268-74
Published 2013-3-8
DOI 10.1016/j.bbrc.2013.01.119
PII S0006-291X(13)00220-9
PMID 23399561
MeSH Adaptor Proteins, Signal Transducing / genetics Adaptor Proteins, Signal Transducing / metabolism* Amino Acid Sequence Animals Autophagy Catalysis Cells, Cultured Cytoskeletal Proteins / metabolism Fibroblasts / metabolism Membrane Proteins / metabolism Mice Mice, Knockout Microtubule-Associated Proteins / metabolism* Molecular Sequence Data Phagosomes Type C Phospholipases / metabolism
IF 2.559
Times Cited 3
WOS Category BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
Mice GFP-LC3#53 (RBRC00806)