論文 - 詳細
| RRC ID | 21927 |
|---|---|
| 著者 | Carvalho M, Schwudke D, Sampaio JL, Palm W, Riezman I, Dey G, Gupta GD, Mayor S, Riezman H, Shevchenko A, Kurzchalia TV, Eaton S. |
| タイトル | Survival strategies of a sterol auxotroph. |
| ジャーナル | Development |
| Abstract |
The high sterol concentration in eukaryotic cell membranes is thought to influence membrane properties such as permeability, fluidity and microdomain formation. Drosophila cannot synthesize sterols, but do require them for development. Does this simply reflect a requirement for sterols in steroid hormone biosynthesis, or is bulk membrane sterol also essential in Drosophila? If the latter is true, how do they survive fluctuations in sterol availability and maintain membrane homeostasis? Here, we show that Drosophila require both bulk membrane sterol and steroid hormones in order to complete adult development. When sterol availability is restricted, Drosophila larvae modulate their growth to maintain membrane sterol levels within tight limits. When dietary sterol drops below a minimal threshold, larvae arrest growth and development in a reversible manner. Strikingly, membrane sterol levels in arrested larvae are dramatically reduced (dropping sixfold on average) in most tissues except the nervous system. Thus, sterols are dispensable for maintaining the basic membrane biophysical properties required for cell viability; these functions can be performed by non-sterol lipids when sterols are unavailable. However, bulk membrane sterol is likely to have essential functions in specific tissues during development. In tissues in which sterol levels drop, the overall level of sphingolipids increases and the proportion of different sphingolipid variants is altered. These changes allow survival, but not growth, when membrane sterol levels are low. This relationship between sterols and sphingolipids could be an ancient and conserved principle of membrane homeostasis. |
| 巻・号 | 137(21) |
| ページ | 3675-85 |
| 公開日 | 2010-11-1 |
| DOI | 10.1242/dev.044560 |
| PII | 137/21/3675 |
| PMID | 20940226 |
| PMC | PMC2964098 |
| MeSH | Animals Animals, Genetically Modified Cell Membrane / metabolism Cell Survival Cells, Cultured Drosophila / embryology Drosophila / growth & development* Drosophila / metabolism* Drosophila / physiology Embryo, Nonmammalian Hormones / metabolism Larva / growth & development Larva / metabolism Models, Biological Sphingolipids / metabolism Steroids / metabolism Sterols / metabolism* Survival / physiology |
| IF | 5.611 |
| 引用数 | 64 |
| WOS 分野 | DEVELOPMENTAL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | 30502R-1 30502R-4 |