RRC ID 2533
Author Takahashi S, Onuma Y, Yokota C, Westmoreland JJ, Asashima M, Wright CV.
Title Nodal-related gene Xnr5 is amplified in the Xenopus genome.
Journal Genesis
Abstract In Xenopus, six nodal-related genes (Xnrs) have been identified to date. We found numerous tandem duplications of Xnr5 in the Xenopus laevis and Xenopus tropicalis genomes that involve highly conserved copies of coding and regulatory regions. The duplicated versions of Xnr5 were expressed in both the superficial and deep layer of dorsal endoderm and in the deep layer of ventral endoderm, where the initial inducers of mesendoderm formation would be expected to be localized. Overexpression of secreted inhibitors of Xnrs led to a substantially enhanced transcription of the duplicated Xnr5 genes and Xnr6 in embryos. Therefore, Xnr5 and Xnr6 have a novel feedback loop to inhibit transcription of Xnr5 and Xnr6. These results suggest that the initialization of a strong Xnr5 and Xnr6 signal is enabled by the rapid transcription from multiple genes. The novel feedback loop may negatively regulate transcription of Xnr5s and Xnr6 to limit overproduction of these potent inducers, with the Xnr5/Xnr6 signal then activating positive (Xnrs) and negative (Xlefty) loops, which regulate the range of mesodermal tissues produced.
Volume 44(7)
Pages 309-21
Published 2006-7
DOI 10.1002/dvg.20217
PMID 16791846
MeSH Animals Base Sequence DNA, Complementary / isolation & purification Embryo, Nonmammalian Gene Amplification* Gene Expression Profiling Gene Expression Regulation Genetic Variation Left-Right Determination Factors Molecular Sequence Data Nodal Signaling Ligands Sequence Homology, Nucleic Acid Transforming Growth Factor beta / metabolism Xenopus Proteins / genetics* Xenopus Proteins / isolation & purification Xenopus laevis / genetics*
IF 1.74
Times Cited 18
WOS Category GENETICS & HEREDITY DEVELOPMENTAL BIOLOGY
Resource
Xenopus tropicalis