RRC ID 27324
Author Mounet F, Moing A, Kowalczyk M, Rohrmann J, Petit J, Garcia V, Maucourt M, Yano K, Deborde C, Aoki K, Bergès H, Granell A, Fernie AR, Bellini C, Rothan C, Lemaire-Chamley M.
Title Down-regulation of a single auxin efflux transport protein in tomato induces precocious fruit development.
Journal J Exp Bot
Abstract The PIN-FORMED (PIN) auxin efflux transport protein family has been well characterized in the model plant Arabidopsis thaliana, where these proteins are crucial for auxin regulation of various aspects of plant development. Recent evidence indicates that PIN proteins may play a role in fruit set and early fruit development in tomato (Solanum lycopersicum), but functional analyses of PIN-silenced plants failed to corroborate this hypothesis. Here it is demonstrated that silencing specifically the tomato SlPIN4 gene, which is predominantly expressed in tomato flower bud and young developing fruit, leads to parthenocarpic fruits due to precocious fruit development before fertilization. This phenotype was associated with only slight modifications of auxin homeostasis at early stages of flower bud development and with minor alterations of ARF and Aux/IAA gene expression. However, microarray transcriptome analysis and large-scale quantitative RT-PCR profiling of transcription factors in developing flower bud and fruit highlighted differentially expressed regulatory genes, which are potential targets for auxin control of fruit set and development in tomato. In conclusion, this work provides clear evidence that the tomato PIN protein SlPIN4 plays a major role in auxin regulation of tomato fruit set, possibly by preventing precocious fruit development in the absence of pollination, and further gives new insights into the target genes involved in fruit set.
Volume 63(13)
Pages 4901-17
Published 2012-8
DOI 10.1093/jxb/ers167
PII ers167
PMID 22844095
PMC PMC3427993
MeSH Biological Transport Down-Regulation Flowers Fruit / cytology Fruit / genetics Fruit / growth & development* Fruit / metabolism Gene Expression Profiling Gene Expression Regulation, Developmental* Gene Expression Regulation, Plant Indoleacetic Acids / metabolism* Lycopersicon esculentum / cytology Lycopersicon esculentum / genetics* Lycopersicon esculentum / growth & development Lycopersicon esculentum / metabolism Oligonucleotide Array Sequence Analysis Phenotype Phylogeny Plant Growth Regulators / metabolism* Plant Proteins / genetics* Plant Proteins / metabolism Plant Roots Plants, Genetically Modified RNA Interference Signal Transduction Transcription Factors / genetics Transcription Factors / metabolism Up-Regulation
IF 5.36
Times Cited 39
WOS Category PLANT SCIENCES
Resource
Tomato