RRC ID 27720
Author Yabushita S, Fukamachi K, Kikuchi F, Ozaki M, Miyata K, Sukata T, Deguchi Y, Tanaka H, Kakehashi A, Kawamura S, Uwagawa S, Wanibuchi H, Suzui M, Alexander DB, Tsuda H.
Title Twenty-one proteins up-regulated in human H-ras oncogene transgenic rat pancreas cancers are up-regulated in human pancreas cancer.
Journal Pancreas
Abstract OBJECTIVES:We have established rat models of pancreatic ductal adenocarcinoma (PDAC) in which expression of a human H-ras(G12V) or K-ras(G12V) oncogene regulated by the Cre/lox system drives pancreatic carcinogenesis. Pancreatic ductal adenocarcinoma which develops in H-ras(G12V) and K-ras(G12V) transgenic rats is cytogenetically and histopathologically similar to human PDAC. The present study was designed to determine the feasibility of using the commercially available H-ras(G12V) transgenic rat to find diagnostic protein biomarkers for human pancreatic cancer.
METHODS:For an animal model to be useful for searching for protein biomarkers for a disease, it is essential that proteins that are up-regulated in the model are also up-regulated in humans. We used liquid chromatography-tandem mass spectrometry (LC-MS/MS) to compare H-ras(G12V) transgenic rat PDAC with surrounding normal pancreas tissue.
RESULTS:We identified 30 up-regulated proteins in the H-ras(G12V) transgenic rat PDAC lesions; importantly, 21 human homologs of these 30 rat proteins are up-regulated in human pancreatic cancer patients.
CONCLUSIONS:These results indicate that numerous proteins that are up-regulated in H-ras(G12V) transgenic rat PDAC are also up-regulated in human pancreatic cancer; therefore, this rat model can be used to search for diagnostic biomarkers for this disease.
Volume 42(6)
Pages 1034-9
Published 2013-8-1
DOI 10.1097/MPA.0b013e3182883624
PMID 23648844
MeSH Adenocarcinoma / genetics Adenocarcinoma / metabolism* Adenocarcinoma / pathology Animals Biomarkers, Tumor / genetics Biomarkers, Tumor / metabolism Carcinoma, Pancreatic Ductal / genetics Carcinoma, Pancreatic Ductal / metabolism* Carcinoma, Pancreatic Ductal / pathology Chromatography, Liquid Disease Models, Animal Feasibility Studies Humans Mutation Pancreatic Neoplasms / genetics Pancreatic Neoplasms / metabolism* Pancreatic Neoplasms / pathology Proteins / genetics Proteins / metabolism* Rats Rats, Transgenic Tandem Mass Spectrometry Up-Regulation ras Proteins / genetics ras Proteins / metabolism*
IF 2.92
Times Cited 5
Rats SD-Tg(CAG-HRAS*G12V)250Htsu(strainID=790)