論文 - 詳細
| RRC ID | 27877 |
|---|---|
| 著者 | Ma N, Liu Q, Zhang L, Henske EP, Ma Y. |
| タイトル | TORC1 signaling is governed by two negative regulators in fission yeast. |
| ジャーナル | Genetics |
| Abstract |
The target of rapamycin (TOR) is a highly conserved protein kinase that regulates cell growth and metabolism. Here we performed a genome-wide screen to identify negative regulators of TOR complex 1 (TORC1) in Schizosaccharomyces pombe by isolating mutants that phenocopy Δtsc2, in which TORC1 signaling is known to be up-regulated. We discovered that Δnpr2 displayed similar phenotypes to Δtsc2 in terms of amino acid uptake defects and mislocalization of the Cat1 permease. However, Δnpr2 and Δtsc2 clearly showed different phenotypes in terms of rapamycin supersensitivity and Isp5 transcription upon various treatments. Furthermore, we showed that Tor2 controls amino acid homeostasis at the transcriptional and post-transcriptional levels. Our data reveal that both Npr2 and Tsc2 negatively regulate TORC1 signaling, and Npr2, but not Tsc2, may be involved in the feedback loop of a nutrient-sensing pathway. |
| 巻・号 | 195(2) |
| ページ | 457-68 |
| 公開日 | 2013-10-1 |
| DOI | 10.1534/genetics.113.154674 |
| PII | genetics.113.154674 |
| PMID | 23934889 |
| PMC | PMC3781973 |
| MeSH | Amino Acid Sequence Amino Acid Transport Systems / metabolism Amino Acids / genetics Amino Acids / metabolism* Cell Cycle Gene Expression Regulation, Fungal Mechanistic Target of Rapamycin Complex 1 Multiprotein Complexes / genetics* Multiprotein Complexes / metabolism Mutation Receptors, Atrial Natriuretic Factor / genetics Schizosaccharomyces / genetics Schizosaccharomyces pombe Proteins / genetics* Schizosaccharomyces pombe Proteins / metabolism Signal Transduction TOR Serine-Threonine Kinases / genetics* TOR Serine-Threonine Kinases / metabolism |
| IF | 4.015 |
| 引用数 | 14 |
| WOS 分野 | GENETICS & HEREDITY |
| リソース情報 | |
| 酵母 | h- leu1-32 tor2-287 |