RRC ID 2797
著者 Taniguchi N, Shinoda Y, Takei N, Nawa H, Ogura A, Tominaga-Yoshino K.
タイトル Possible involvement of BDNF release in long-lasting synapse formation induced by repetitive PKA activation.
ジャーナル Neurosci Lett
Abstract For the analysis of the cellular mechanism underlying long-term synaptic plasticity, a model system that allows long-lasting pursuit is required. Previously we reported that, in hippocampal neurons under dissociated cell culture conditions, repeated (but not a single) transient activation of protein kinase A (PKA) led to an increase in the number of synapses that lasted >3 weeks, and hence we proposed that this phenomenon should serve as an appropriate model system. Here we report that repeated pulsatile application of brain-derived neurotrophic factor (BDNF) leads to persistent synapse formation equivalent to that after the repeated transient activation of PKA. A BDNF-scavenging substance applied concomitantly with PKA activation abolished the synapse formation. The release of BDNF upon PKA activation was confirmed by phosphorylation of TrkB. These results indicate that the release of BDNF is involved in the putative signaling cascade connecting PKA activation and synapse formation.
巻・号 406(1-2)
ページ 38-42
公開日 2006-10-2
DOI 10.1016/j.neulet.2006.06.071
PII S0304-3940(06)00638-0
PMID 16904263
MeSH Animals Biomarkers / metabolism Brain-Derived Neurotrophic Factor / metabolism* Brain-Derived Neurotrophic Factor / pharmacology Cell Differentiation / drug effects Cell Differentiation / physiology Cells, Cultured Cyclic AMP / analogs & derivatives Cyclic AMP / metabolism Cyclic AMP / pharmacology Cyclic AMP-Dependent Protein Kinases / drug effects Cyclic AMP-Dependent Protein Kinases / metabolism* Disks Large Homolog 4 Protein Enzyme Activation / drug effects Enzyme Activation / physiology Hippocampus / cytology Hippocampus / metabolism* Intracellular Signaling Peptides and Proteins / metabolism Membrane Proteins / metabolism Neural Pathways / cytology Neural Pathways / drug effects Neural Pathways / metabolism* Neuronal Plasticity / drug effects Neuronal Plasticity / physiology* Phosphorylation / drug effects Presynaptic Terminals / drug effects Presynaptic Terminals / metabolism Presynaptic Terminals / ultrastructure Rats Rats, Wistar Receptor, trkB / metabolism Synapses / drug effects Synapses / metabolism* Synapses / ultrastructure Synaptophysin / metabolism Thionucleotides / pharmacology
IF 2.274
引用数 16
WOS 分野 NEUROSCIENCES
リソース情報
ラット