RRC ID |
28082
|
Author |
Morante J, Vallejo DM, Desplan C, Dominguez M.
|
Title |
Conserved miR-8/miR-200 defines a glial niche that controls neuroepithelial expansion and neuroblast transition.
|
Journal |
Dev Cell
|
Abstract |
Neuroepithelial cell proliferation must be carefully balanced with the transition to neuroblast (neural stem cell) to control neurogenesis. Here, we show that loss of the Drosophila microRNA mir-8 (the homolog of vertebrate miR-200 family) results in both excess proliferation and ectopic neuroblast transition. Unexpectedly, mir-8 is expressed in a subpopulation of optic-lobe-associated cortex glia that extend processes that ensheath the neuroepithelium, suggesting that glia cells communicate with the neuroepithelium. We provide evidence that miR-8-positive glia express Spitz, a transforming growth factor α (TGF-α)-like ligand that triggers epidermal growth factor receptor (EGFR) activation to promote neuroepithelial proliferation and neuroblast formation. Further, our experiments suggest that miR-8 ensures both a correct glial architecture and the spatiotemporal control of Spitz protein synthesis via direct binding to Spitz 3' UTR. Together, these results establish glial-derived cues as key regulatory elements in the control of neuroepithelial cell proliferation and the neuroblast transition.
|
Volume |
27(2)
|
Pages |
174-187
|
Published |
2013-10-28
|
DOI |
10.1016/j.devcel.2013.09.018
|
PII |
S1534-5807(13)00544-3
|
PMID |
24139822
|
PMC |
PMC3931912
|
MeSH |
3' Untranslated Regions
Animals
Cell Differentiation / genetics
Cell Proliferation
Drosophila Proteins / metabolism*
Drosophila melanogaster / genetics
Drosophila melanogaster / growth & development*
Drosophila melanogaster / metabolism
Enzyme Activation
Epidermal Growth Factor / metabolism*
ErbB Receptors / metabolism
Membrane Proteins / metabolism*
MicroRNAs / genetics*
Neural Stem Cells
Neuroepithelial Cells / metabolism
Neurogenesis
Neuroglia / cytology
Neuroglia / metabolism
Optic Lobe, Nonmammalian / cytology
Optic Lobe, Nonmammalian / growth & development*
Optic Lobe, Nonmammalian / metabolism
Receptors, Invertebrate Peptide / metabolism
Signal Transduction / genetics
|
IF |
10.092
|
Times Cited |
36
|
WOS Category
|
DEVELOPMENTAL BIOLOGY
CELL BIOLOGY
|
Resource |
Drosophila |
|