論文 - 詳細
RRC ID | 28082 |
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著者 | Morante J, Vallejo DM, Desplan C, Dominguez M. |
タイトル | Conserved miR-8/miR-200 defines a glial niche that controls neuroepithelial expansion and neuroblast transition. |
ジャーナル | Dev Cell |
Abstract |
Neuroepithelial cell proliferation must be carefully balanced with the transition to neuroblast (neural stem cell) to control neurogenesis. Here, we show that loss of the Drosophila microRNA mir-8 (the homolog of vertebrate miR-200 family) results in both excess proliferation and ectopic neuroblast transition. Unexpectedly, mir-8 is expressed in a subpopulation of optic-lobe-associated cortex glia that extend processes that ensheath the neuroepithelium, suggesting that glia cells communicate with the neuroepithelium. We provide evidence that miR-8-positive glia express Spitz, a transforming growth factor α (TGF-α)-like ligand that triggers epidermal growth factor receptor (EGFR) activation to promote neuroepithelial proliferation and neuroblast formation. Further, our experiments suggest that miR-8 ensures both a correct glial architecture and the spatiotemporal control of Spitz protein synthesis via direct binding to Spitz 3' UTR. Together, these results establish glial-derived cues as key regulatory elements in the control of neuroepithelial cell proliferation and the neuroblast transition. |
巻・号 | 27(2) |
ページ | 174-187 |
公開日 | 2013-10-28 |
DOI | 10.1016/j.devcel.2013.09.018 |
PII | S1534-5807(13)00544-3 |
PMID | 24139822 |
PMC | PMC3931912 |
MeSH | 3' Untranslated Regions Animals Cell Differentiation / genetics Cell Proliferation Drosophila Proteins / metabolism* Drosophila melanogaster / genetics Drosophila melanogaster / growth & development* Drosophila melanogaster / metabolism Enzyme Activation Epidermal Growth Factor / metabolism* ErbB Receptors / metabolism Membrane Proteins / metabolism* MicroRNAs / genetics* Neural Stem Cells Neuroepithelial Cells / metabolism Neurogenesis Neuroglia / cytology Neuroglia / metabolism Optic Lobe, Nonmammalian / cytology Optic Lobe, Nonmammalian / growth & development* Optic Lobe, Nonmammalian / metabolism Receptors, Invertebrate Peptide / metabolism Signal Transduction / genetics |
IF | 10.092 |
引用数 | 36 |
WOS 分野 | DEVELOPMENTAL BIOLOGY CELL BIOLOGY |
リソース情報 | |
ショウジョウバエ |