RRC ID 28082
著者 Morante J, Vallejo DM, Desplan C, Dominguez M.
タイトル Conserved miR-8/miR-200 defines a glial niche that controls neuroepithelial expansion and neuroblast transition.
ジャーナル Dev Cell
Abstract Neuroepithelial cell proliferation must be carefully balanced with the transition to neuroblast (neural stem cell) to control neurogenesis. Here, we show that loss of the Drosophila microRNA mir-8 (the homolog of vertebrate miR-200 family) results in both excess proliferation and ectopic neuroblast transition. Unexpectedly, mir-8 is expressed in a subpopulation of optic-lobe-associated cortex glia that extend processes that ensheath the neuroepithelium, suggesting that glia cells communicate with the neuroepithelium. We provide evidence that miR-8-positive glia express Spitz, a transforming growth factor α (TGF-α)-like ligand that triggers epidermal growth factor receptor (EGFR) activation to promote neuroepithelial proliferation and neuroblast formation. Further, our experiments suggest that miR-8 ensures both a correct glial architecture and the spatiotemporal control of Spitz protein synthesis via direct binding to Spitz 3' UTR. Together, these results establish glial-derived cues as key regulatory elements in the control of neuroepithelial cell proliferation and the neuroblast transition.
巻・号 27(2)
ページ 174-187
公開日 2013-10-28
DOI 10.1016/j.devcel.2013.09.018
PII S1534-5807(13)00544-3
PMID 24139822
PMC PMC3931912
MeSH 3' Untranslated Regions Animals Cell Differentiation / genetics Cell Proliferation Drosophila Proteins / metabolism* Drosophila melanogaster / genetics Drosophila melanogaster / growth & development* Drosophila melanogaster / metabolism Enzyme Activation Epidermal Growth Factor / metabolism* ErbB Receptors / metabolism Membrane Proteins / metabolism* MicroRNAs / genetics* Neural Stem Cells Neuroepithelial Cells / metabolism Neurogenesis Neuroglia / cytology Neuroglia / metabolism Optic Lobe, Nonmammalian / cytology Optic Lobe, Nonmammalian / growth & development* Optic Lobe, Nonmammalian / metabolism Receptors, Invertebrate Peptide / metabolism Signal Transduction / genetics
IF 10.092
引用数 36
WOS 分野 DEVELOPMENTAL BIOLOGY CELL BIOLOGY
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