Reference - Detail
| RRC ID | 29011 |
|---|---|
| Author | Brock DA, Gomer RH. |
| Title | A cell-counting factor regulating structure size in Dictyostelium. |
| Journal | Genes Dev |
| Abstract |
Developing Dictyostelium cells form large aggregation streams that break up into groups of 0.2 x 10(5) to 1 x 10(5) cells. Each group then becomes a fruiting body. smlA cells oversecrete an unknown factor that causes aggregation streams to break up into groups of approximately 5 x 10(3) cells and thus form very small fruiting bodies. We have purified the counting factor and find that it behaves as a complex of polypeptides with an effective molecular mass of 450 kD. One of the polypeptides is a 40-kD hydrophilic protein we have named counting. In transformants with a disrupted counting gene, there is no detectable secretion of counting factor, and the aggregation streams do not break up, resulting in huge (up to 2 x 10(5) cell) fruiting bodies. |
| Volume | 13(15) |
| Pages | 1960-9 |
| Published | 1999-8-1 |
| DOI | 10.1101/gad.13.15.1960 |
| PMID | 10444594 |
| PMC | PMC316923 |
| MeSH | Amino Acid Sequence Animals Base Sequence Cell Aggregation Cell Count Cloning, Molecular Culture Media, Conditioned Dictyostelium / cytology* Dictyostelium / genetics Dictyostelium / growth & development* Molecular Sequence Data Molecular Weight Mutation Phenotype Protein Sorting Signals / genetics Protozoan Proteins / chemistry Protozoan Proteins / genetics Protozoan Proteins / isolation & purification Protozoan Proteins / metabolism* RNA, Messenger / genetics RNA, Messenger / metabolism Sequence Analysis Signal Transduction |
| IF | 9.527 |
| Times Cited | 93 |
| WOS Category | GENETICS & HEREDITY CELL BIOLOGY DEVELOPMENTAL BIOLOGY |
| Resource | |
| Cellular slime molds | S00324 |