RRC ID 29068
著者 Muramoto T, Takeda S, Furuya Y, Urushihara H.
タイトル Reverse genetic analyses of gamete-enriched genes revealed a novel regulator of the cAMP signaling pathway in Dictyostelium discoideum.
ジャーナル Mech Dev
Abstract Sexual development in Dictyostelium discoideum is initiated by the fusion of opposite mating type cells to form zygote giant cells, which subsequently gather and phagocytose surrounding cells for nutrition to form macrocysts. Here we performed the targeting of 24 highly gamete-enriched genes we previously isolated, and successfully generated knockout mutants for 16 genes and RNAi mutants for 20 genes including 6 genes without disruptants. In the knockout mutants of two genes, cell aggregation toward the giant cells was much less extensive and many cells remained around poorly formed macrocysts. We named these genes tmcB and tmcC. Although macrocyst formation of wild type cells was suppressed by the addition of exogenous cAMP, that of knockout mutants of tmcB was much less sensitive. The mRNA level of phosphodiesterase (pde) was higher and that of its inhibitor (pdi) was lower in the latter cells compared to the parental strains during sexual development. Thus, tmcB appeared to be a novel regulator of the cAMP signaling pathway specific to sexual development. Knockout mutants of tmcC were indistinguishable from the wild type cells with respect to the cAMP response, suggesting that this gene is relevant to other processes.
巻・号 122(5)
ページ 733-43
公開日 2005-5-1
DOI 10.1016/j.mod.2004.11.015
PII S0925-4773(04)00281-3
PMID 15817229
MeSH Amino Acid Sequence Animals Animals, Genetically Modified Cyclic AMP / metabolism* Dictyostelium / metabolism* Dose-Response Relationship, Drug Genetic Techniques* Intracellular Signaling Peptides and Proteins / genetics* Intracellular Signaling Peptides and Proteins / physiology Models, Genetic Molecular Sequence Data Mutagenesis Mutation RNA / metabolism RNA Interference Reverse Transcriptase Polymerase Chain Reaction Signal Transduction Time Factors
IF 2.126
引用数 11
WOS 分野 DEVELOPMENTAL BIOLOGY
リソース情報
細胞性粘菌 S00252 S00258 S00259 S00260 S00261 S00263 S00264 S00265 S00266 S00267 S00268 S00269 S00270 S00271 S00272 S00273 S00274 S00275 S00276 S00277 S00278 S00279 S00280 S90143 S90144 S90157 S90158 S90159 S90160 S90268 S90269 S90375 S90376 S90001 S90002 S90003 S90004 S90005