論文 - 詳細
| RRC ID | 2932 |
|---|---|
| 著者 | Handa Y, Suzuki M, Ohya K, Iwai H, Ishijima N, Koleske AJ, Fukui Y, Sasakawa C. |
| タイトル | Shigella IpgB1 promotes bacterial entry through the ELMO-Dock180 machinery. |
| ジャーナル | Nat Cell Biol |
| Abstract |
Shigella use a special mechanism to invade epithelial cells called 'the trigger mechanism of entry', which allows epithelial cells to trap several bacteria simultaneously. On contact, Shigella deliver effectors into epithelial cells through the type III secretion system. Here, we show that one of the effectors, IpgB1, has a pivotal role in producing membrane ruffles by exploiting the RhoG-ELMO-Dock180 pathway to stimulate Rac1 activity. Using pulldown assays, we identified engulfment and cell motility (ELMO) protein as the IpgB1 binding partner. IpgB1 colocalized with ELMO and Dock180 in membrane ruffles induced by Shigella. Shigella invasiveness and IpgB1-induced ruffles were less in ELMO- and Dock180-knockdown cells compared with wild-type cells. Membrane association of ELMO-Dock180 with ruffles were promoted when cells expressed an IpgB1-ELMO chimera, establishing that IpgB1 mimics the role of RhoG in producing membrane ruffles. Taken together, our findings show that IpgB1 mimicry is the key to invasion by Shigella. |
| 巻・号 | 9(1) |
| ページ | 121-8 |
| 公開日 | 2007-1-1 |
| DOI | 10.1038/ncb1526 |
| PII | ncb1526 |
| PMID | 17173036 |
| MeSH | Adaptor Proteins, Signal Transducing / genetics Adaptor Proteins, Signal Transducing / metabolism* Animals Bacterial Adhesion* Cell Line Cell Membrane / metabolism Dogs HeLa Cells Humans Immunoprecipitation Mice Models, Biological NIH 3T3 Cells Protein Transport RNA Interference Shigella / pathogenicity* Shigella / physiology Signal Transduction Transduction, Genetic Transfection rac GTP-Binding Proteins / genetics rac GTP-Binding Proteins / metabolism* rac1 GTP-Binding Protein / metabolism* rac1 GTP-Binding Protein / physiology |
| IF | 20.042 |
| 引用数 | 106 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | F1000Research |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 病原微生物 | NA |