Reference - Detail
| RRC ID | 29500 |
|---|---|
| Author | Fukunishi K, Miyakubi K, Hatanaka M, Otsuru N, Hirata A, Shimoda C, Nakamura T. |
| Title | The fission yeast spore is coated by a proteinaceous surface layer comprising mainly Isp3. |
| Journal | Mol Biol Cell |
| Abstract |
The spore is a dormant cell that is resistant to various environmental stresses. As compared with the vegetative cell wall, the spore wall has a more extensive structure that confers resistance on spores. In the fission yeast Schizosaccharomyces pombe, the polysaccharides glucan and chitosan are major components of the spore wall; however, the structure of the spore surface remains unknown. We identify the spore coat protein Isp3/Meu4. The isp3 disruptant is viable and executes meiotic nuclear divisions as efficiently as the wild type, but isp3∆ spores show decreased tolerance to heat, digestive enzymes, and ethanol. Electron microscopy shows that an electron-dense layer is formed at the outermost region of the wild-type spore wall. This layer is not observed in isp3∆ spores. Furthermore, Isp3 is abundantly detected in this layer by immunoelectron microscopy. Thus Isp3 constitutes the spore coat, thereby conferring resistance to various environmental stresses. |
| Volume | 25(10) |
| Pages | 1549-59 |
| Published | 2014-5-1 |
| DOI | 10.1091/mbc.E13-12-0731 |
| PII | mbc.E13-12-0731 |
| PMID | 24623719 |
| PMC | PMC4019487 |
| MeSH | Amidohydrolases Amino Acid Sequence Cell Wall / chemistry Cell Wall / enzymology Cell Wall / genetics* Chitin / biosynthesis Chitin Synthase / genetics* Chitosan / metabolism Ethanol / toxicity Green Fluorescent Proteins / genetics Hot Temperature / adverse effects Meiosis / genetics Microscopy, Immunoelectron Molecular Chaperones / biosynthesis Molecular Chaperones / genetics* Phosphoproteins / biosynthesis Phosphoproteins / genetics* Proteolysis Schizosaccharomyces / cytology* Schizosaccharomyces pombe Proteins / biosynthesis Schizosaccharomyces pombe Proteins / genetics* Schizosaccharomyces pombe Proteins / metabolism Spores, Fungal / cytology* Stress, Physiological |
| IF | 3.791 |
| Times Cited | 5 |
| WOS Category | CELL BIOLOGY |
| Resource | |
| Yeast | FY7520 FY17852 FY17853 FY17854 FY17858 FY17862 FY17873 FY17875 FY14016 FY17731 FY7456 FY7816 FY7051 BY19600 |