論文 - 詳細
| RRC ID | 29793 |
|---|---|
| 著者 | Nakahigashi K, Nishimura K, Miyamoto K, Inokuchi H. |
| タイトル | Photosensitivity of a protoporphyrin-accumulating, light-sensitive mutant (visA) of Escherichia coli K-12. |
| ジャーナル | Proc Natl Acad Sci U S A |
| Abstract |
Mutations in the visA gene of Escherichia coli cause the mutant bacteria to die upon illumination with visible light. We confirmed genetically that the visA gene is a structural gene for ferrochelatase (protoheme ferro-lyase, EC 4.99.1.1). Since other mutations in the genes involved in the biosynthesis of heme can cure the photosensitivity, the light-induced cell death appears to be brought about by the accumulation of protoporphyrin IX, one of the substrates of ferrochelatase. When cells are illuminated with visible light, protoporphyrin IX seems to produce an active species of oxygen (probably 1O2) that is harmful to the cells. This defect is the same as that associated with the human disease protoporphyria. |
| 巻・号 | 88(23) |
| ページ | 10520-4 |
| 公開日 | 1991-12-1 |
| DOI | 10.1073/pnas.88.23.10520 |
| PMID | 1835790 |
| PMC | PMC52960 |
| MeSH | Anaerobiosis Bacteriophage lambda / genetics Catalase / metabolism Chromosome Deletion Cloning, Molecular DNA, Bacterial / genetics DNA, Bacterial / isolation & purification Escherichia coli / genetics* Escherichia coli / physiology Escherichia coli / radiation effects Ferrochelatase / genetics* Ferrochelatase / metabolism Genes, Bacterial* Genotype Light Methylnitronitrosoguanidine / pharmacology Mutagenesis Phenotype Protoporphyrins / metabolism* Recombinant Proteins / metabolism beta-Galactosidase / genetics beta-Galactosidase / metabolism |
| IF | 9.412 |
| 引用数 | 66 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | IH91 IH71 IH72 |