RRC ID 30164
著者 Nakano H, Goto S, Nakayashiki T, Inokuchi H.
タイトル Temperature-sensitive mutations in various genes of Escherichia coli K12 can be suppressed by the ssrA gene for 10Sa RNA (tmRNA).
ジャーナル Mol Genet Genomics
Abstract An Escherichia coli strain with a deletion in the ssrA gene that encodes 10Sa RNA (tmRNA) was used to screen for temperature-sensitive (ts) mutants whose ts phenotypes were suppressible by introduction of the wild-type ssrA gene. Mutants in four different genes were isolated. Ts mutants of this type were also obtained in a screen for mutations in thyA, the structural gene for thymidylate synthase. The ThyA activity in crude extracts prepared from the ts mutants was temperature-sensitive. The presence of the ssrA gene caused an increase in the total amount of the temperature-sensitive enzyme expressed, rather than suppressing the ts activity of the enzyme itself. SsrA-DD, a mutant form of 10Sa RNA, suppressed the ts phenotype of a thyA mutant, suggesting that degradation of a tagged peptide was not required for suppression of the ts phenotype. Considering the fact that ssrA-suppressible mutants could be isolated as temperature-sensitive mutants with mutations in different genes, it seems evident that trans-translation can occur on mRNA that is not lacking its stop codon.
巻・号 265(4)
ページ 615-21
公開日 2001-6-1
DOI 10.1007/s004380100453
PMID 11459181
MeSH Amino Acid Substitution Bacterial Proteins / genetics Codon, Terminator Escherichia coli / genetics* Gene Expression Regulation, Bacterial* Genes, Bacterial* Genetic Complementation Test Mutation* Phenotype Point Mutation Protein Biosynthesis RNA, Bacterial / genetics RNA, Bacterial / physiology* Ribose-Phosphate Pyrophosphokinase / genetics Sequence Deletion Suppression, Genetic* Temperature Thymidylate Synthase / genetics
IF 2.797
引用数 14
WOS 分野 GENETICS & HEREDITY BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
原核生物(大腸菌) IH39 IH40 IH41 IH42