| RRC ID |
301
|
| Author |
Kaneuchi T, Togawa T, Matsuo T, Fuyama Y, Aigaki T.
|
| Title |
Efficient measurement of H2O2 resistance in Drosophila using an activity monitor.
|
| Journal |
Biogerontology
|
| Abstract |
We have established a method for the efficient measurement of oxidative stress resistance in Drosophila melanogaster, using a commercially available activity monitor. Conditions under which flies in glass tubes placed in the monitor can survive over one month at 25 degrees C were optimized. The active periods of flies were reduced by administration of H(2)O(2) into the media in a dose-dependent manner. Although we used only eight flies per assay, far fewer individuals than in conventional methods, it was possible to detect the effects of H(2)O(2) at a statistically significant level. Increased levels of H(2)O(2) resistance were confirmed in transgenic flies overexpressing antioxidant enzymes, catalase or Cu/Zn superoxide dismutase. We applied the method to determine oxidative stress resistance in fly lines bearing insertions of a gene misexpression vector. H(2)O(2) resistance in these flies varied considerably depending on the insertion, and positively correlated with previously determined longevity. We identified one insertion that conferred a significantly higher level of resistance to H(2)O(2) compared to controls. Molecular analysis of the insertion revealed that a misexpressed transcript matched an expressed sequence tag, and suggested that its full-length product was overproduced upon GAL4 activation. Our method should be applicable to the systematic screening for genes involved in the antioxidant mechanism in Drosophila.
|
| Volume |
4(3)
|
| Pages |
157-65
|
| Published |
2003-5-1
|
| DOI |
10.1023/a:1024145822785
|
| PII |
5120781
|
| PMID |
12815315
|
| MeSH |
Aging / genetics
Aging / metabolism
Animals
Animals, Genetically Modified
Antioxidants / metabolism
Behavior, Animal
Catalase / genetics
Drosophila melanogaster / genetics*
Drosophila melanogaster / metabolism
Hydrogen Peroxide / pharmacology*
Mutation
Oxidants / pharmacology*
Oxidative Stress / drug effects
Oxidative Stress / genetics*
Superoxide Dismutase / genetics
|
| IF |
3.667
|
| Times Cited |
8
|
|
WOS Category
|
GERIATRICS & GERONTOLOGY
|
| Resource |
| Drosophila |
|
