論文 - 詳細
| RRC ID | 30207 |
|---|---|
| 著者 | Minoura I, Hachikubo Y, Yamakita Y, Takazaki H, Ayukawa R, Uchimura S, Muto E. |
| タイトル | Overexpression, purification, and functional analysis of recombinant human tubulin dimer. |
| ジャーナル | FEBS Lett |
| Abstract |
Microtubules consisting of tubulin dimers play essential roles in various cellular functions. Investigating the structure-function relationship of tubulin dimers requires a method to prepare sufficient quantities of recombinant tubulin. To this end, we simultaneously expressed human α1- and β3-tubulin using a baculovirus-insect cell expression system that enabled the purification of 5mg recombinant tubulin per litre of cell culture. The purified recombinant human tubulin could be polymerized into microtubules that glide on a kinesin-coated glass surface. The method provides a powerful tool for in vitro functional analyses of microtubules. |
| 巻・号 | 587(21) |
| ページ | 3450-5 |
| 公開日 | 2013-11-1 |
| DOI | 10.1016/j.febslet.2013.08.032 |
| PII | S0014-5793(13)00661-3 |
| PMID | 24021646 |
| MeSH | Cells, Cultured Humans Kinesins / metabolism Protein Multimerization Recombinant Proteins / genetics* Recombinant Proteins / isolation & purification* Recombinant Proteins / metabolism Tubulin / genetics* Tubulin / isolation & purification* Tubulin / metabolism |
| IF | 3.057 |
| 引用数 | 50 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | F1000Research |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | pFBD-Hs alpha1CGH/beta3CGF (RDB12753) pFBD-Hs alpha1(K40R)CGH/beta3CGF (RDB12754). |