| RRC ID |
30214
|
| Author |
Yokoo R, Zawadzki KA, Nabeshima K, Drake M, Arur S, Villeneuve AM.
|
| Title |
COSA-1 reveals robust homeostasis and separable licensing and reinforcement steps governing meiotic crossovers.
|
| Journal |
Cell
|
| Abstract |
Crossovers (COs) between homologous chromosomes ensure their faithful segregation during meiosis. We identify C. elegans COSA-1, a cyclin-related protein conserved in metazoa, as a key component required to convert meiotic double-strand breaks (DSBs) into COs. During late meiotic prophase, COSA-1 localizes to foci that correspond to the single CO site on each homolog pair and indicate sites of eventual concentration of other conserved CO proteins. Chromosomes gain and lose competence to load CO proteins during meiotic progression, with competence to load COSA-1 requiring prior licensing. Our data further suggest a self-reinforcing mechanism maintaining CO designation. Modeling of a nonlinear dose-response relationship between IR-induced DSBs and COSA-1 foci reveals efficient conversion of DSBs into COs when DSBs are limiting and a robust capacity to limit cytologically differentiated CO sites when DSBs are in excess. COSA-1 foci serve as a unique live cell readout for investigating CO formation and CO interference.
|
| Volume |
149(1)
|
| Pages |
75-87
|
| Published |
2012-3-30
|
| DOI |
10.1016/j.cell.2012.01.052
|
| PII |
S0092-8674(12)00286-3
|
| PMID |
22464324
|
| PMC |
PMC3339199
|
| MeSH |
Animals
Caenorhabditis elegans / cytology*
Caenorhabditis elegans / genetics
Caenorhabditis elegans / metabolism
Caenorhabditis elegans Proteins / genetics
Caenorhabditis elegans Proteins / metabolism*
Chromosomes / metabolism
Crossing Over, Genetic*
Cyclins / genetics
Cyclins / metabolism*
DNA Breaks, Double-Stranded
DNA-Binding Proteins / genetics
DNA-Binding Proteins / metabolism*
Meiosis*
Models, Molecular
Mutation
|
| IF |
38.637
|
| Times Cited |
105
|
|
WOS Category
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| Resource |
| C.elegans |
tm3298 |
