RRC ID 30427
Author Oda H, Tsukita S.
Title Dynamic features of adherens junctions during Drosophila embryonic epithelial morphogenesis revealed by a Dalpha-catenin-GFP fusion protein.
Journal Dev. Genes Evol.
Abstract Cell-cell adherens junctions (AJs), comprised of the cadherin-catenin adhesion system, contribute to cell shape changes and cell movements in epithelial morphogenesis. However, little is known about the dynamic features of AJs in cells of the developing embryo. In this study, we constructed Dalpha-catenin fused with a green fluorescent protein (Dalpha-catenin-GFP), and found that it targeted apically located AJ-based contacts but not other lateral contacts in epithelial cells of living Drosophila embryos. Using time-lapse fluorescence microscopy, we examined the dynamic performance of AJs containing Dalpha-catenin-GFP in epithelial morphogenetic movements. In the ventral ectoderm of stage 11 embryos, concentration and deconcentration of Dalpha-catenin-GFP occurred concomitantly with changes in length of AJ contacts. In the lateral ectoderm of embryos at the same stage, dynamic behaviour of AJs was concerted with division and delamination of sensory organ precursor (SOP) cells. Moreover, changes in patterns of AJ networks during tracheal extension could be followed. Finally, we utilized Dalpha-catenin-GFP to precisely observe the defects in tracheal fusion in shotgun mutants. Thus, the Dalpha-catenin-GFP fusion protein is a helpful tool to simultaneously observe morphogenetic movements and AJ dynamics at high spatio-temporal resolution.
Volume 209(4)
Pages 218-25
Published 1999-4
PMID 10079365
MeSH Animals Armadillo Domain Proteins Cadherins / metabolism Cell Adhesion Cell Differentiation Cell Division Cytoskeletal Proteins / analysis* Cytoskeletal Proteins / genetics Drosophila / cytology Drosophila / embryology* Drosophila / genetics Drosophila Proteins* Epithelium / chemistry Epithelium / embryology Epithelium / metabolism Fluorescent Antibody Technique Gene Expression Regulation, Developmental Green Fluorescent Proteins Insect Proteins / physiology Intercellular Junctions / chemistry* Luminescent Proteins / analysis* Luminescent Proteins / genetics Microscopy, Confocal Mutation Phenotype Proto-Oncogene Proteins / physiology Recombinant Fusion Proteins / analysis Recombinant Fusion Proteins / genetics Sense Organs / chemistry Sense Organs / cytology Sense Organs / embryology Signal Transduction Trachea / chemistry Trachea / embryology Trachea / metabolism Trans-Activators* Transcription Factors Wnt1 Protein alpha Catenin
IF 2.125
Times Cited 53
WOS Category EVOLUTIONARY BIOLOGY CELL BIOLOGY DEVELOPMENTAL BIOLOGY
Resource
Drosophila DGRC#109001