| RRC ID |
30469
|
| Author |
Schuldiner O, Berdnik D, Levy JM, Wu JS, Luginbuhl D, Gontang AC, Luo L.
|
| Title |
piggyBac-based mosaic screen identifies a postmitotic function for cohesin in regulating developmental axon pruning.
|
| Journal |
Dev Cell
|
| Abstract |
Developmental axon pruning is widely used to refine neural circuits. We performed a mosaic screen to identify mutations affecting axon pruning of Drosophila mushroom body gamma neurons. We constructed a modified piggyBac vector with improved mutagenicity and generated insertions in >2000 genes. We identified two cohesin subunits (SMC1 and SA) as being essential for axon pruning. The cohesin complex maintains sister-chromatid cohesion during cell division in eukaryotes. However, we show that the pruning phenotype in SMC1(-/-) clones is rescued by expressing SMC1 in neurons, revealing a postmitotic function. SMC1(-/-) clones exhibit reduced levels of the ecdysone receptor EcR-B1, a key regulator of axon pruning. The pruning phenotype is significantly suppressed by overexpressing EcR-B1 and is enhanced by a reduced dose of EcR, supporting a causal relationship. We also demonstrate a postmitotic role for SMC1 in dendrite targeting of olfactory projection neurons. We suggest that cohesin regulates diverse aspects of neuronal morphogenesis.
|
| Volume |
14(2)
|
| Pages |
227-38
|
| Published |
2008-2-1
|
| DOI |
10.1016/j.devcel.2007.11.001
|
| PII |
S1534-5807(07)00419-4
|
| PMID |
18267091
|
| PMC |
PMC2268086
|
| MeSH |
Alleles
Animals
Axons / metabolism*
Cell Cycle Proteins / metabolism*
Cell Proliferation
Chromosomal Proteins, Non-Histone / metabolism*
DNA Transposable Elements / genetics*
Dendrites / metabolism
Drosophila Proteins / metabolism
Drosophila melanogaster / cytology*
Drosophila melanogaster / growth & development*
Genetic Markers
Mitosis*
Mosaicism*
Mushroom Bodies / cytology
Mutagenesis, Insertional
Mutation / genetics
Nuclear Proteins / metabolism*
Olfactory Pathways / metabolism
Phenotype
Receptors, Steroid / metabolism
Transgenes
|
| IF |
10.092
|
| Times Cited |
167
|
|
WOS Category
|
DEVELOPMENTAL BIOLOGY
CELL BIOLOGY
|
| Resource |
| Drosophila |
DGRC#140001 |
