Reference - Detail
| RRC ID | 30469 |
|---|---|
| Author | Schuldiner O, Berdnik D, Levy JM, Wu JS, Luginbuhl D, Gontang AC, Luo L. |
| Title | piggyBac-based mosaic screen identifies a postmitotic function for cohesin in regulating developmental axon pruning. |
| Journal | Dev Cell |
| Abstract |
Developmental axon pruning is widely used to refine neural circuits. We performed a mosaic screen to identify mutations affecting axon pruning of Drosophila mushroom body gamma neurons. We constructed a modified piggyBac vector with improved mutagenicity and generated insertions in >2000 genes. We identified two cohesin subunits (SMC1 and SA) as being essential for axon pruning. The cohesin complex maintains sister-chromatid cohesion during cell division in eukaryotes. However, we show that the pruning phenotype in SMC1(-/-) clones is rescued by expressing SMC1 in neurons, revealing a postmitotic function. SMC1(-/-) clones exhibit reduced levels of the ecdysone receptor EcR-B1, a key regulator of axon pruning. The pruning phenotype is significantly suppressed by overexpressing EcR-B1 and is enhanced by a reduced dose of EcR, supporting a causal relationship. We also demonstrate a postmitotic role for SMC1 in dendrite targeting of olfactory projection neurons. We suggest that cohesin regulates diverse aspects of neuronal morphogenesis. |
| Volume | 14(2) |
| Pages | 227-38 |
| Published | 2008-2-1 |
| DOI | 10.1016/j.devcel.2007.11.001 |
| PII | S1534-5807(07)00419-4 |
| PMID | 18267091 |
| PMC | PMC2268086 |
| MeSH | Alleles Animals Axons / metabolism* Cell Cycle Proteins / metabolism* Cell Proliferation Chromosomal Proteins, Non-Histone / metabolism* DNA Transposable Elements / genetics* Dendrites / metabolism Drosophila Proteins / metabolism Drosophila melanogaster / cytology* Drosophila melanogaster / growth & development* Genetic Markers Mitosis* Mosaicism* Mushroom Bodies / cytology Mutagenesis, Insertional Mutation / genetics Nuclear Proteins / metabolism* Olfactory Pathways / metabolism Phenotype Receptors, Steroid / metabolism Transgenes |
| IF | 10.092 |
| Times Cited | 167 |
| WOS Category | DEVELOPMENTAL BIOLOGY CELL BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
|
| Total number of mentions | 0 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Drosophila | DGRC#140001 |