RRC ID 30595
Author Nakamura S, Watakabe I, Nishimura T, Toyoda A, Taniguchi Y, Tanaka M.
Title Analysis of medaka sox9 orthologue reveals a conserved role in germ cell maintenance.
Journal PLoS ONE
Abstract The sex determining gene is divergent among different animal species. However, sox9 is up-regulated in the male gonads in a number of species in which it is the essential regulator of testis determination. It is therefore often discussed that the sex determining gene-sox9 axis functions in several vertebrates. In our current study, we show that sox9b in the medaka (Oryzias latipes) is one of the orthologues of mammalian Sox9 at syntenic and expression levels. Medaka sox9b affects the organization of extracellular matrices, which represents a conserved role of sox9, but does not directly regulate testis determination. We made this determination via gene expression and phenotype analyses of medaka with different copy numbers of sox9b. Sox9b is involved in promoting cellular associations and is indispensible for the proper proliferation and survival of germ cells in both female and male medaka gonads. Medaka mutants that lack sox9b function exhibit a seemingly paradoxical phenotype of sex reversal to male. This is explained by a reduction in the germ cell number associated with aberrant extracellular matrices. Together with its identified roles in other vertebrate gonads, a testis-determining role for Sox9 in mammals is likely to have been neofunctionalized and appended to its conserved role in germ cell maintenance.
Volume 7(1)
Pages e29982
Published 2012
DOI 10.1371/journal.pone.0029982
PII PONE-D-11-18251
PMID 22253846
PMC PMC3257256
MeSH Alleles Animals Apoptosis / genetics Base Sequence Cell Count Cell Proliferation Cell Survival Conserved Sequence* Extracellular Matrix / metabolism Female Gene Expression Regulation Germ Cells / cytology* Germ Cells / metabolism* Heterozygote Male Molecular Sequence Data Mutation / genetics Oryzias / genetics Oryzias / metabolism* Phenotype SOX9 Transcription Factor / chemistry* SOX9 Transcription Factor / genetics SOX9 Transcription Factor / metabolism* Sequence Homology, Amino Acid Sex Determination Processes / genetics Sex Differentiation / genetics Synteny / genetics Testis / cytology Testis / metabolism
IF 2.776
Times Cited 56
WOS Category DEVELOPMENTAL BIOLOGY
Resource
Medaka cDNA sox9bK16X sox9bK136X TILLING_MUTANT MT830 MT436 Hatching enzyme