RRC ID |
30595
|
著者 |
Nakamura S, Watakabe I, Nishimura T, Toyoda A, Taniguchi Y, Tanaka M.
|
タイトル |
Analysis of medaka sox9 orthologue reveals a conserved role in germ cell maintenance.
|
ジャーナル |
PLoS One
|
Abstract |
The sex determining gene is divergent among different animal species. However, sox9 is up-regulated in the male gonads in a number of species in which it is the essential regulator of testis determination. It is therefore often discussed that the sex determining gene-sox9 axis functions in several vertebrates. In our current study, we show that sox9b in the medaka (Oryzias latipes) is one of the orthologues of mammalian Sox9 at syntenic and expression levels. Medaka sox9b affects the organization of extracellular matrices, which represents a conserved role of sox9, but does not directly regulate testis determination. We made this determination via gene expression and phenotype analyses of medaka with different copy numbers of sox9b. Sox9b is involved in promoting cellular associations and is indispensible for the proper proliferation and survival of germ cells in both female and male medaka gonads. Medaka mutants that lack sox9b function exhibit a seemingly paradoxical phenotype of sex reversal to male. This is explained by a reduction in the germ cell number associated with aberrant extracellular matrices. Together with its identified roles in other vertebrate gonads, a testis-determining role for Sox9 in mammals is likely to have been neofunctionalized and appended to its conserved role in germ cell maintenance.
|
巻・号 |
7(1)
|
ページ |
e29982
|
公開日 |
2012-1-1
|
DOI |
10.1371/journal.pone.0029982
|
PII |
PONE-D-11-18251
|
PMID |
22253846
|
PMC |
PMC3257256
|
MeSH |
Alleles
Animals
Apoptosis / genetics
Base Sequence
Cell Count
Cell Proliferation
Cell Survival
Conserved Sequence*
Extracellular Matrix / metabolism
Female
Gene Expression Regulation
Germ Cells / cytology*
Germ Cells / metabolism*
Heterozygote
Male
Molecular Sequence Data
Mutation / genetics
Oryzias / genetics
Oryzias / metabolism*
Phenotype
SOX9 Transcription Factor / chemistry*
SOX9 Transcription Factor / genetics
SOX9 Transcription Factor / metabolism*
Sequence Homology, Amino Acid
Sex Determination Processes / genetics
Sex Differentiation / genetics
Synteny / genetics
Testis / cytology
Testis / metabolism
|
IF |
2.74
|
引用数 |
56
|
WOS 分野
|
DEVELOPMENTAL BIOLOGY
|
リソース情報 |
メダカ |
cDNA
sox9bK16X
sox9bK136X
TILLING_MUTANT
MT830
MT436
Hatching enzyme |