論文 - 詳細
| RRC ID | 30764 |
|---|---|
| 著者 | Kansup J, Tsugama D, Liu S, Takano T. |
| タイトル | The Arabidopsis adaptor protein AP-3μ interacts with the G-protein β subunit AGB1 and is involved in abscisic acid regulation of germination and post-germination development. |
| ジャーナル | J Exp Bot |
| Abstract |
Heterotrimeric G-proteins (G-proteins) have been implicated in ubiquitous signalling mechanisms in eukaryotes. In plants, G-proteins modulate hormonal and stress responses and regulate diverse developmental processes. However, the molecular mechanisms of their functions are largely unknown. A yeast two-hybrid screen was performed to identify interacting partners of the Arabidopsis G-protein β subunit AGB1. One of the identified AGB1-interacting proteins is the Arabidopsis adaptor protein AP-3µ. The interaction between AGB1 and AP-3µ was confirmed by an in vitro pull-down assay and bimolecular fluorescence complementation assay. Two ap-3µ T-DNA insertional mutants were found to be hyposensitive to abscisic acid (ABA) during germination and post-germination growth, whereas agb1 mutants were hypersensitive to ABA. During seed germination, agb1/ap-3µ double mutants were more sensitive to ABA than the wild type but less sensitive than agb1 mutants. However, in post-germination growth, the double mutants were as sensitive to ABA as agb1 mutants. These data suggest that AP-3µ positively regulates the ABA responses independently of AGB1 in seed germination, while AP-3µ does require AGB1 to regulate ABA responses during post-germination growth. |
| 巻・号 | 64(18) |
| ページ | 5611-21 |
| 公開日 | 2013-12-1 |
| DOI | 10.1093/jxb/ert327 |
| PII | ert327 |
| PMID | 24098050 |
| PMC | PMC3871816 |
| MeSH | Abscisic Acid / metabolism* Abscisic Acid / pharmacology Adaptor Proteins, Signal Transducing / genetics Adaptor Proteins, Signal Transducing / metabolism* Arabidopsis / genetics Arabidopsis / growth & development* Arabidopsis / metabolism Arabidopsis Proteins / drug effects Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism* GTP-Binding Protein beta Subunits / drug effects GTP-Binding Protein beta Subunits / genetics GTP-Binding Protein beta Subunits / metabolism* Gene Expression Regulation, Plant Germination / physiology* Mutation Phenotype |
| IF | 5.908 |
| 引用数 | 10 |
| WOS 分野 | PLANT SCIENCES |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| シロイヌナズナ / 植物培養細胞・遺伝子 | pda08829 |