論文 - 詳細
| RRC ID | 30832 |
|---|---|
| 著者 | Maday S, Holzbaur EL. |
| タイトル | Autophagosome biogenesis in primary neurons follows an ordered and spatially regulated pathway. |
| ジャーナル | Dev Cell |
| Abstract |
Autophagy is an essential degradative pathway in neurons, yet little is known about mechanisms driving autophagy in highly polarized cells. Here, we use dual-color live-cell imaging to investigate the neuron-specific mechanisms of constitutive autophagosome biogenesis in primary dorsal root ganglion (DRG) and hippocampal cultures. Under basal conditions, autophagosomes are continuously generated in the axon tip. There is an ordered assembly of proteins recruited with stereotypical kinetics onto the developing autophagosome. Plasma- or mitochondrial-derived membranes were not incorporated into nascent autophagosomes in the distal axon. Rather, autophagosomes are generated at double FYVE-containing protein 1 (DFCP1)-positive subdomains of the endoplasmic reticulum (ER), distinct from ER exit sites. Biogenesis events are enriched distally; autophagosomes form infrequently in dendrites, the soma, or midaxon, consistent with a compartmentalized pathway for constitutive autophagy in primary neurons. Distal biogenesis may facilitate degradation of damaged mitochondria and long-lived cytoplasmic proteins reaching the axon tip via slow axonal transport. |
| 巻・号 | 30(1) |
| ページ | 71-85 |
| 公開日 | 2014-7-14 |
| DOI | 10.1016/j.devcel.2014.06.001 |
| PII | S1534-5807(14)00345-1 |
| PMID | 25026034 |
| PMC | PMC4109719 |
| MeSH | Animals Autophagy* Axons / metabolism Biological Transport Cell Membrane / metabolism Cells, Cultured Dendritic Cells / cytology Dendritic Cells / metabolism Endoplasmic Reticulum / metabolism* Hippocampus / cytology Hippocampus / metabolism* Membrane Proteins / metabolism* Mice Mice, Transgenic Mitochondria / metabolism Neurons / cytology Neurons / metabolism* Phagosomes / physiology* |
| IF | 10.092 |
| 引用数 | 143 |
| WOS 分野 | DEVELOPMENTAL BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 7 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 実験動物マウス | RBRC00806 |