RRC ID 30900
Author Velu TJ, Beguinot L, Vass WC, Willingham MC, Merlino GT, Pastan I, Lowy DR.
Title Epidermal-growth-factor-dependent transformation by a human EGF receptor proto-oncogene.
Journal Science
Abstract The epidermal growth factor (EGF) receptor gene EGFR has been placed in a retrovirus vector to examine the growth properties of cells that experimentally overproduce a full-length EGF receptor. NIH 3T3 cells transfected with the viral DNA or infected with the corresponding rescued retrovirus developed a fully transformed phenotype in vitro that required both functional EGFR expression and the presence of EGF in the growth medium. Cells expressing 4 x 10(5) EGF receptors formed tumors in nude mice, while control cells did not. Therefore, the EGFR retrovirus, which had a titer on NIH 3T3 cells that was greater than 10(7) focus-forming units per milliliter, can efficiently transfer and express this gene, and increased numbers of EGF receptors can contribute to the transformed phenotype.
Volume 238(4832)
Pages 1408-10
Published 1987-12-4
DOI 10.1126/science.3500513
PMID 3500513
MeSH Animals Cell Transformation, Neoplastic / chemically induced Cell Transformation, Neoplastic / genetics* Cells, Cultured DNA, Recombinant Epidermal Growth Factor / pharmacology* ErbB Receptors / drug effects ErbB Receptors / genetics* Fibroblasts / pathology Genetic Vectors Harvey murine sarcoma virus / genetics Humans Male Mice Mice, Nude Neoplasms, Experimental / etiology Proto-Oncogenes* Recombinant Proteins / genetics
IF 41.846
Times Cited 436
DNA material pco12 EGFR (RDB01276)