論文 - 詳細
| RRC ID | 30900 |
|---|---|
| 著者 | Velu TJ, Beguinot L, Vass WC, Willingham MC, Merlino GT, Pastan I, Lowy DR. |
| タイトル | Epidermal-growth-factor-dependent transformation by a human EGF receptor proto-oncogene. |
| ジャーナル | Science |
| Abstract |
The epidermal growth factor (EGF) receptor gene EGFR has been placed in a retrovirus vector to examine the growth properties of cells that experimentally overproduce a full-length EGF receptor. NIH 3T3 cells transfected with the viral DNA or infected with the corresponding rescued retrovirus developed a fully transformed phenotype in vitro that required both functional EGFR expression and the presence of EGF in the growth medium. Cells expressing 4 x 10(5) EGF receptors formed tumors in nude mice, while control cells did not. Therefore, the EGFR retrovirus, which had a titer on NIH 3T3 cells that was greater than 10(7) focus-forming units per milliliter, can efficiently transfer and express this gene, and increased numbers of EGF receptors can contribute to the transformed phenotype. |
| 巻・号 | 238(4832) |
| ページ | 1408-10 |
| 公開日 | 1987-12-4 |
| DOI | 10.1126/science.3500513 |
| PMID | 3500513 |
| MeSH | Animals Cell Transformation, Neoplastic / chemically induced Cell Transformation, Neoplastic / genetics* Cells, Cultured DNA, Recombinant Epidermal Growth Factor / pharmacology* ErbB Receptors / drug effects ErbB Receptors / genetics* Fibroblasts / pathology Genetic Vectors Harvey murine sarcoma virus / genetics Humans Male Mice Mice, Nude Neoplasms, Experimental / etiology Proto-Oncogene Mas Proto-Oncogenes* Recombinant Proteins / genetics |
| IF | 41.846 |
| 引用数 | 436 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 17 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 3.0 |
| リソース情報 | |
| 遺伝子材料 | pco12 EGFR (RDB01276) |