RRC ID 30910
Author Shirahama-Noda K, Kira S, Yoshimori T, Noda T.
Title TRAPPIII is responsible for vesicular transport from early endosomes to Golgi, facilitating Atg9 cycling in autophagy.
Journal J Cell Sci
Abstract Autophagy is a bulk protein-degradation process that is regulated by many factors. In this study, we quantitatively assessed the contribution of each essential yeast gene to autophagy. Of the contributing factors that we identified, we focused on the TRAPPIII complex, which was recently shown to act as a guanine-nucleotide exchange factor for the Rab small GTPase Ypt1. Autophagy is defective in the TRAPPIII mutant under nutrient-rich conditions (Cvt pathway), but starvation-induced autophagy is only partially affected. Here, we show that TRAPPIII functions at the Golgi complex to receive general retrograde vesicle traffic from early endosomes. Cargo proteins in this TRAPPIII-dependent pathway include Atg9, a transmembrane protein that is essential for autophagy, and Snc1, a SNARE unrelated to autophagy. When cells were starved, further disruption of vesicle movement from late endosomes to the Golgi caused defects in Atg9 trafficking and autophagy. Thus, TRAPPIII-dependent sorting pathways provide Atg9 reservoirs for pre-autophagosomal structure and phagophore assembly sites under nutrient-rich conditions, whereas the late endosome-to-Golgi pathway is added to these reservoirs when nutrients are limited. This clarification of the role of TRAPPIII elucidates how general membrane traffic contributes to autophagy.
Volume 126(Pt 21)
Pages 4963-73
Published 2013-11-1
DOI 10.1242/jcs.131318
PII jcs.131318
PMID 23986483
MeSH Autophagy* Autophagy-Related Proteins Endosomes / genetics Endosomes / metabolism* Golgi Apparatus / genetics Golgi Apparatus / metabolism* Membrane Proteins / genetics Membrane Proteins / metabolism* Protein Binding Protein Transport Saccharomyces cerevisiae / cytology* Saccharomyces cerevisiae / genetics Saccharomyces cerevisiae / metabolism* Saccharomyces cerevisiae Proteins / genetics Saccharomyces cerevisiae Proteins / metabolism* Transport Vesicles / metabolism Vesicular Transport Proteins / genetics Vesicular Transport Proteins / metabolism*
IF 4.517
Times Cited 54
Yeast pNHK53 pMK43