論文 - 詳細
| RRC ID | 30910 |
|---|---|
| 著者 | Shirahama-Noda K, Kira S, Yoshimori T, Noda T. |
| タイトル | TRAPPIII is responsible for vesicular transport from early endosomes to Golgi, facilitating Atg9 cycling in autophagy. |
| ジャーナル | J Cell Sci |
| Abstract |
Autophagy is a bulk protein-degradation process that is regulated by many factors. In this study, we quantitatively assessed the contribution of each essential yeast gene to autophagy. Of the contributing factors that we identified, we focused on the TRAPPIII complex, which was recently shown to act as a guanine-nucleotide exchange factor for the Rab small GTPase Ypt1. Autophagy is defective in the TRAPPIII mutant under nutrient-rich conditions (Cvt pathway), but starvation-induced autophagy is only partially affected. Here, we show that TRAPPIII functions at the Golgi complex to receive general retrograde vesicle traffic from early endosomes. Cargo proteins in this TRAPPIII-dependent pathway include Atg9, a transmembrane protein that is essential for autophagy, and Snc1, a SNARE unrelated to autophagy. When cells were starved, further disruption of vesicle movement from late endosomes to the Golgi caused defects in Atg9 trafficking and autophagy. Thus, TRAPPIII-dependent sorting pathways provide Atg9 reservoirs for pre-autophagosomal structure and phagophore assembly sites under nutrient-rich conditions, whereas the late endosome-to-Golgi pathway is added to these reservoirs when nutrients are limited. This clarification of the role of TRAPPIII elucidates how general membrane traffic contributes to autophagy. |
| 巻・号 | 126(Pt 21) |
| ページ | 4963-73 |
| 公開日 | 2013-11-1 |
| DOI | 10.1242/jcs.131318 |
| PII | jcs.131318 |
| PMID | 23986483 |
| MeSH | Autophagy* Autophagy-Related Proteins Endosomes / genetics Endosomes / metabolism* Golgi Apparatus / genetics Golgi Apparatus / metabolism* Membrane Proteins / genetics Membrane Proteins / metabolism* Protein Binding Protein Transport Saccharomyces cerevisiae / cytology* Saccharomyces cerevisiae / genetics Saccharomyces cerevisiae / metabolism* Saccharomyces cerevisiae Proteins / genetics Saccharomyces cerevisiae Proteins / metabolism* Transport Vesicles / metabolism Vesicular Transport Proteins / genetics Vesicular Transport Proteins / metabolism* |
| IF | 4.573 |
| 引用数 | 54 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | F1000Research |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 酵母 | pNHK53 pMK43 |