RRC ID 30987
Author Xie F, Murray JD, Kim J, Heckmann AB, Edwards A, Oldroyd GE, Downie JA.
Title Legume pectate lyase required for root infection by rhizobia.
Journal Proc. Natl. Acad. Sci. U.S.A.
Abstract To allow rhizobial infection of legume roots, plant cell walls must be locally degraded for plant-made infection threads (ITs) to be formed. Here we identify a Lotus japonicus nodulation pectate lyase gene (LjNPL), which is induced in roots and root hairs by rhizobial nodulation (Nod) factors via activation of the nodulation signaling pathway and the NIN transcription factor. Two Ljnpl mutants produced uninfected nodules and most infections arrested as infection foci in root hairs or roots. The few partially infected nodules that did form contained large abnormal infections. The purified LjNPL protein had pectate lyase activity, demonstrating that this activity is required for rhizobia to penetrate the cell wall and initiate formation of plant-made infection threads. Therefore, we conclude that legume-determined degradation of plant cell walls is required for root infection during initiation of the symbiotic interaction between rhizobia and legumes.
Volume 109(2)
Pages 633-8
Published 2012-1-10
DOI 10.1073/pnas.1113992109
PII 1113992109
PMID 22203959
PMC PMC3258600
MeSH Amino Acid Sequence Base Sequence Enzyme Induction / physiology Likelihood Functions Loteae / enzymology* Loteae / growth & development Loteae / microbiology* Mesorhizobium / genetics* Mesorhizobium / metabolism Models, Genetic Molecular Sequence Data Mutation / genetics Phylogeny* Polysaccharide-Lyases / biosynthesis* Polysaccharide-Lyases / genetics* Reverse Transcriptase Polymerase Chain Reaction Root Nodules, Plant / microbiology*
IF 9.504
Times Cited 69
WOS Category PLANT SCIENCES
Resource
Lotus / Glycine pUB-GW-GFP