論文 - 詳細
| RRC ID | 30987 |
|---|---|
| 著者 | Xie F, Murray JD, Kim J, Heckmann AB, Edwards A, Oldroyd GE, Downie JA. |
| タイトル | Legume pectate lyase required for root infection by rhizobia. |
| ジャーナル | Proc Natl Acad Sci U S A |
| Abstract |
To allow rhizobial infection of legume roots, plant cell walls must be locally degraded for plant-made infection threads (ITs) to be formed. Here we identify a Lotus japonicus nodulation pectate lyase gene (LjNPL), which is induced in roots and root hairs by rhizobial nodulation (Nod) factors via activation of the nodulation signaling pathway and the NIN transcription factor. Two Ljnpl mutants produced uninfected nodules and most infections arrested as infection foci in root hairs or roots. The few partially infected nodules that did form contained large abnormal infections. The purified LjNPL protein had pectate lyase activity, demonstrating that this activity is required for rhizobia to penetrate the cell wall and initiate formation of plant-made infection threads. Therefore, we conclude that legume-determined degradation of plant cell walls is required for root infection during initiation of the symbiotic interaction between rhizobia and legumes. |
| 巻・号 | 109(2) |
| ページ | 633-8 |
| 公開日 | 2012-1-10 |
| DOI | 10.1073/pnas.1113992109 |
| PII | 1113992109 |
| PMID | 22203959 |
| PMC | PMC3258600 |
| MeSH | Amino Acid Sequence Base Sequence Enzyme Induction / physiology Likelihood Functions Lotus / enzymology* Lotus / growth & development Lotus / microbiology* Mesorhizobium / genetics* Mesorhizobium / metabolism Models, Genetic Molecular Sequence Data Mutation / genetics Phylogeny* Polysaccharide-Lyases / biosynthesis* Polysaccharide-Lyases / genetics* Reverse Transcriptase Polymerase Chain Reaction Root Nodules, Plant / microbiology* |
| IF | 9.412 |
| 引用数 | 111 |
| WOS 分野 | PLANT SCIENCES |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 12 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ミヤコグサ・ダイズ | pUB-GW-GFP |