RRC ID 3103
Author Yin XJ, Volk S, Ljung K, Mehlmer N, Dolezal K, Ditengou F, Hanano S, Davis SJ, Schmelzer E, Sandberg G, Teige M, Palme K, Pickart C, Bachmair A.
Title Ubiquitin lysine 63 chain forming ligases regulate apical dominance in Arabidopsis.
Journal Plant Cell
Abstract Lys-63-linked multiubiquitin chains play important roles in signal transduction in yeast and in mammals, but the functions for this type of chain in plants remain to be defined. The RING domain protein RGLG2 (for RING domain Ligase2) from Arabidopsis thaliana can be N-terminally myristoylated and localizes to the plasma membrane. It can form Lys-63-linked multiubiquitin chains in an in vitro reaction. RGLG2 has overlapping functions with its closest sequelog, RGLG1, and single mutants in either gene are inconspicuous. rglg1 rglg2 double mutant plants exhibit loss of apical dominance and altered phyllotaxy, two traits critically influenced by the plant hormone auxin. Auxin and cytokinin levels are changed, and the plants show a decreased response to exogenously added auxin. Changes in the abundance of PIN family auxin transport proteins and synthetic lethality with a mutation in the auxin transport regulator BIG suggest that the directional flow of auxin is modulated by RGLG activity. Modification of proteins by Lys-63-linked multiubiquitin chains is thus important for hormone-regulated, basic plant architecture.
Volume 19(6)
Pages 1898-911
Published 2007-6
DOI 10.1105/tpc.107.052035
PII tpc.107.052035
PMID 17586653
PMC PMC1955712
MeSH Arabidopsis / drug effects Arabidopsis / enzymology* Arabidopsis / genetics Arabidopsis / growth & development* Biological Transport / drug effects Catalysis / drug effects Cytokinins / metabolism Enzyme Activation / drug effects Gene Expression Regulation, Plant / drug effects Genes, Plant Indoleacetic Acids / metabolism Lysine / metabolism* Membrane Proteins / metabolism Meristem / drug effects Mutation / genetics Myristic Acid / pharmacology Phenotype Plant Leaves / cytology Plant Leaves / drug effects Plant Shoots / drug effects Plant Shoots / growth & development* Protein Transport / drug effects Recombinant Fusion Proteins / metabolism Two-Hybrid System Techniques Ubiquitin-Protein Ligases / metabolism*
IF 8.228
Times Cited 46
WOS Category PLANT SCIENCES BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
Resource
Arabidopsis / Cultured plant cells, genes pda08576