Reference - Detail
|Author||Tsuda M, Ootaka R, Ohkura C, Kishita Y, Seong KH, Matsuo T, Aigaki T.|
|Title||Loss of Trx-2 enhances oxidative stress-dependent phenotypes in Drosophila.|
Overexpression of thioredoxin (TRX) confers oxidative stress resistance and extends lifespan in mammals and insects. However, less is known about phenotypes associated with loss of TRX. We investigated loss-of-function phenotypes of Trx-2 in Drosophila, and found that the mutant flies are hyper-susceptible to paraquat, a free radical generator, but not to hydrogen peroxide. They contain a high amount of protein carbonyl, which dramatically increases with age. Trx-2 mutants express high levels of anti-oxidant genes, such as superoxide dismutase, catalase, and glutathione synthetase. This is the first demonstration of biochemical and physiological consequences caused by loss of Trx-2 in Drosophila.
|MeSH||Animals Drosophila Proteins / genetics Drosophila Proteins / metabolism* Drosophila melanogaster / drug effects Drosophila melanogaster / genetics Drosophila melanogaster / metabolism* Gene Expression Regulation / drug effects Insecticide Resistance / drug effects Insecticide Resistance / genetics Longevity / drug effects Motor Activity / drug effects Mutation / genetics Oxidants / metabolism Oxidative Stress* / drug effects Oxidative Stress* / genetics Paraquat / toxicity Phenotype Protein Carbonylation / drug effects Thioredoxins / genetics Thioredoxins / metabolism*|
|WOS Category||BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY|