RRC ID 31386
Author Tsuda M, Ootaka R, Ohkura C, Kishita Y, Seong KH, Matsuo T, Aigaki T.
Title Loss of Trx-2 enhances oxidative stress-dependent phenotypes in Drosophila.
Journal FEBS Lett.
Abstract Overexpression of thioredoxin (TRX) confers oxidative stress resistance and extends lifespan in mammals and insects. However, less is known about phenotypes associated with loss of TRX. We investigated loss-of-function phenotypes of Trx-2 in Drosophila, and found that the mutant flies are hyper-susceptible to paraquat, a free radical generator, but not to hydrogen peroxide. They contain a high amount of protein carbonyl, which dramatically increases with age. Trx-2 mutants express high levels of anti-oxidant genes, such as superoxide dismutase, catalase, and glutathione synthetase. This is the first demonstration of biochemical and physiological consequences caused by loss of Trx-2 in Drosophila.
Volume 584(15)
Pages 3398-401
Published 2010-8-4
DOI 10.1016/j.febslet.2010.06.034
PII S0014-5793(10)00529-6
PMID 20600005
MeSH Animals Drosophila Proteins / genetics Drosophila Proteins / metabolism* Drosophila melanogaster / drug effects Drosophila melanogaster / genetics Drosophila melanogaster / metabolism* Gene Expression Regulation / drug effects Insecticide Resistance / drug effects Insecticide Resistance / genetics Longevity / drug effects Motor Activity / drug effects Mutation / genetics Oxidants / metabolism Oxidative Stress* / drug effects Oxidative Stress* / genetics Paraquat / toxicity Phenotype Protein Carbonylation / drug effects Thioredoxins / genetics Thioredoxins / metabolism*
IF 2.675
Times Cited 19