RRC ID 32637
Author Epting D, Slanchev K, Boehlke C, Hoff S, Loges NT, Yasunaga T, Indorf L, Nestel S, Lienkamp SS, Omran H, Kuehn EW, Ronneberger O, Walz G, Kramer-Zucker A.
Title The Rac1 regulator ELMO controls basal body migration and docking in multiciliated cells through interaction with Ezrin.
Journal Development
Abstract Cilia are microtubule-based organelles that are present on most cells and are required for normal tissue development and function. Defective cilia cause complex syndromes with multiple organ manifestations termed ciliopathies. A crucial step during ciliogenesis in multiciliated cells (MCCs) is the association of future basal bodies with the apical plasma membrane, followed by their correct spacing and planar orientation. Here, we report a novel role for ELMO-DOCK1, which is a bipartite guanine nucleotide exchange factor complex for the small GTPase Rac1, and for the membrane-cytoskeletal linker Ezrin, in regulating centriole/basal body migration, docking and spacing. Downregulation of each component results in ciliopathy-related phenotypes in zebrafish and disrupted ciliogenesis in Xenopus epidermal MCCs. Subcellular analysis revealed a striking impairment of basal body docking and spacing, which is likely to account for the observed phenotypes. These results are substantiated by showing a genetic interaction between elmo1 and ezrin b. Finally, we provide biochemical evidence that the ELMO-DOCK1-Rac1 complex influences Ezrin phosphorylation and thereby probably serves as an important molecular switch. Collectively, we demonstrate that the ELMO-Ezrin complex orchestrates ciliary basal body migration, docking and positioning in vivo.
Volume 142(1)
Pages 174-84
Published 2015-1-1
DOI 10.1242/dev.112250
PII 142/1/174
PMID 25516973
MeSH Adaptor Proteins, Signal Transducing / metabolism* Animals Axoneme / metabolism Axoneme / ultrastructure Basal Bodies / metabolism* Cell Membrane / metabolism Cilia / metabolism* Cilia / ultrastructure Cytoskeletal Proteins / metabolism* Embryo, Nonmammalian / metabolism Embryo, Nonmammalian / ultrastructure Membrane Proteins / metabolism Microfilament Proteins / metabolism Models, Biological Phosphorylation Protein Binding Xenopus Proteins / metabolism* Xenopus laevis Zebrafish / embryology Zebrafish Proteins / metabolism* rac GTP-Binding Proteins rac1 GTP-Binding Protein / metabolism*
IF 5.413
Times Cited 11
WOS Category DEVELOPMENTAL BIOLOGY
Resource
DNA material RDB6727 X. laevis whole embryo(stage 10.5) EST clones (XL151H21)
Xenopus tropicalis