Reference - Detail
| RRC ID | 32799 |
|---|---|
| Author | Hasan AU, Ohmori K, Konishi K, Igarashi J, Hashimoto T, Kamitori K, Yamaguchi F, Tsukamoto I, Uyama T, Ishihara Y, Noma T, Tokuda M, Kohno M. |
| Title | Eicosapentaenoic acid upregulates VEGF-A through both GPR120 and PPARγ mediated pathways in 3T3-L1 adipocytes. |
| Journal | Mol Cell Endocrinol |
| Abstract |
Vascular endothelial growth factor-A (VEGF-A) released from adipocytes promotes angiogenesis; and thereby ameliorates the local hypoxia-induced adipose inflammation and insulin resistance. Here, we newly found that eicosapentaenoic acid (EPA) upregulated both mRNA expression and release of VEGF-A in mature 3T3-L1 adipocytes. Silencing mRNA of G-protein coupled receptor 120 (GPR120) and specific inhibition of peroxisome proliferator-activated receptor γ (PPARγ) by GW9662 respectively attenuated the EPA-induced augmentation of VEGF-A release by adipocytes. Furthermore, transfection of GPR120 gene alone and PPARγ gene alone to HEK293 cells respectively increased the promoter activity of VEGF-A as assessed by luciferase reporter assay, which was further augmented when both genes were co-transfected. Promoter deletion analysis and chromatin immunoprecipitation assay revealed that co-transfection of GPR120 enhanced EPA-induced PPARγ binding to PPAR-response element in VEGF-A promoter region. Thus, by the synchronized activation of a membrane receptor GRP120 and a nuclear receptor PPARγ, EPA enhances VEGF-A production in adipocytes. |
| Volume | 406 |
| Pages | 10-8 |
| Published | 2015-5-5 |
| DOI | 10.1016/j.mce.2015.02.012 |
| PII | S0303-7207(15)00088-X |
| PMID | 25697344 |
| MeSH | 3T3-L1 Cells Adipocytes / drug effects Adipocytes / metabolism* Animals Eicosapentaenoic Acid / pharmacology* HEK293 Cells Humans Hypoxia-Inducible Factor 1, alpha Subunit / metabolism Mice Models, Biological PPAR gamma / metabolism* Phosphatidylinositol 3-Kinases / metabolism Protein Binding / drug effects Protein Kinase C / metabolism Receptors, G-Protein-Coupled / metabolism* Response Elements / genetics Signal Transduction / drug effects* Up-Regulation / drug effects* Vascular Endothelial Growth Factor A / genetics* Vascular Endothelial Growth Factor A / metabolism |
| IF | 3.871 |
| Times Cited | 38 |
| WOS Category | ENDOCRINOLOGY & METABOLISM CELL BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 5 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| DNA material | pGL4-phVEGFA (RDB07681) |