Reference - Detail
| RRC ID | 33279 |
|---|---|
| Author | Kaneko T, Sakuma T, Yamamoto T, Mashimo T. |
| Title | Simple knockout by electroporation of engineered endonucleases into intact rat embryos. |
| Journal | Sci Rep |
| Abstract |
Engineered endonucleases, such as zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated (Cas) system, provide a powerful approach for genome editing in animals. However, the microinjection of endonucleases into embryos requires a high skill level, is time consuming, and may cause damage to embryos. Here, we demonstrate that the electroporation of endonuclease mRNAs into intact embryos can induce editing at targeted loci and efficiently produce knockout rats. It is noteworthy that the electroporation of ZFNs resulted in an embryonic survival rate (91%) and a genome-editing rate (73%) that were more than 2-fold higher than the corresponding rates from conventional microinjection. Electroporation technology provides a simple and effective method to produce knockout animals. |
| Volume | 4 |
| Pages | 6382 |
| Published | 2014-10-1 |
| DOI | 10.1038/srep06382 |
| PII | srep06382 |
| PMID | 25269785 |
| PMC | PMC4180828 |
| MeSH | Animals CRISPR-Cas Systems* Cells, Cultured Clustered Regularly Interspaced Short Palindromic Repeats / genetics* Electroporation / methods* Embryo Transfer Embryo, Mammalian / cytology Embryo, Mammalian / metabolism* Endodeoxyribonucleases / genetics Endodeoxyribonucleases / metabolism* Female Gene Targeting / methods* Genetic Engineering / methods* Male Rats Rats, Inbred F344 Zinc Fingers |
| IF | 3.998 |
| Times Cited | 82 |
| WOS Category | MULTIDISCIPLINARY SCIENCES |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 33 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Rats | F344/Stm(strainID=266) |
| Human and Animal Cells | Rat-1(RCB1830) |