Reference - Detail
| RRC ID | 3349 |
|---|---|
| Author | Maita N, Anzai T, Aoyagi H, Mizuno H, Fujiwara H. |
| Title | Crystal structure of the endonuclease domain encoded by the telomere-specific long interspersed nuclear element, TRAS1. |
| Journal | J Biol Chem |
| Abstract |
The telomere-specific long interspersed nuclear element, TRAS1, encodes an endonuclease domain, TRAS1-EN, which specifically cleaves the telomeric repeat targets (TTAGG)n of insects and (TTAGGG)n of vertebrates. To elucidate the sequence-specific recognition properties of TRAS1-EN, we determined the crystal structure at 2.4-A resolution. TRAS1-EN has a four-layered alpha/beta sandwich structure; its topology is similar to apurinic/apyrimidinic endonucleases, but the beta-hairpin (beta10-beta11) at the edge of the DNA-binding surface makes an extra loop that distinguishes TRAS1-EN from cellular apurinic/apyrimidinic endonucleases. A protein-DNA complex model suggests that the beta10-beta11 hairpin fits into the minor groove, enabling interaction with the telomeric repeats. Mutational studies of TRAS1-EN also indicated that the Asp-130 and beta10-beta11 hairpin structure are involved in specific recognition of telomeric repeats. |
| Volume | 279(39) |
| Pages | 41067-76 |
| Published | 2004-9-24 |
| DOI | 10.1074/jbc.M406556200 |
| PII | S0021-9258(20)77205-4 |
| PMID | 15247245 |
| MeSH | Amino Acid Sequence Aspartic Acid / chemistry Base Sequence Catalytic Domain Crystallography, X-Ray / methods DNA / chemistry DNA Mutational Analysis Electrons Endonucleases / chemistry* Humans Long Interspersed Nucleotide Elements* Magnesium / chemistry Models, Molecular Molecular Sequence Data Mutagenesis, Site-Directed Nucleic Acid Conformation* Oligonucleotides / chemistry Plasmids / metabolism Protein Binding Protein Conformation Protein Structure, Secondary Protein Structure, Tertiary Sequence Homology, Amino Acid Telomere / chemistry Telomere / ultrastructure |
| IF | 4.238 |
| Times Cited | 18 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY |
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 11 |
| Altmetric score changes over past 6months | 3.0 |
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