| RRC ID |
3349
|
| 著者 |
Maita N, Anzai T, Aoyagi H, Mizuno H, Fujiwara H.
|
| タイトル |
Crystal structure of the endonuclease domain encoded by the telomere-specific long interspersed nuclear element, TRAS1.
|
| ジャーナル |
J Biol Chem
|
| Abstract |
The telomere-specific long interspersed nuclear element, TRAS1, encodes an endonuclease domain, TRAS1-EN, which specifically cleaves the telomeric repeat targets (TTAGG)n of insects and (TTAGGG)n of vertebrates. To elucidate the sequence-specific recognition properties of TRAS1-EN, we determined the crystal structure at 2.4-A resolution. TRAS1-EN has a four-layered alpha/beta sandwich structure; its topology is similar to apurinic/apyrimidinic endonucleases, but the beta-hairpin (beta10-beta11) at the edge of the DNA-binding surface makes an extra loop that distinguishes TRAS1-EN from cellular apurinic/apyrimidinic endonucleases. A protein-DNA complex model suggests that the beta10-beta11 hairpin fits into the minor groove, enabling interaction with the telomeric repeats. Mutational studies of TRAS1-EN also indicated that the Asp-130 and beta10-beta11 hairpin structure are involved in specific recognition of telomeric repeats.
|
| 巻・号 |
279(39)
|
| ページ |
41067-76
|
| 公開日 |
2004-9-24
|
| DOI |
10.1074/jbc.M406556200
|
| PII |
S0021-9258(20)77205-4
|
| PMID |
15247245
|
| MeSH |
Amino Acid Sequence
Aspartic Acid / chemistry
Base Sequence
Catalytic Domain
Crystallography, X-Ray / methods
DNA / chemistry
DNA Mutational Analysis
Electrons
Endonucleases / chemistry*
Humans
Long Interspersed Nucleotide Elements*
Magnesium / chemistry
Models, Molecular
Molecular Sequence Data
Mutagenesis, Site-Directed
Nucleic Acid Conformation*
Oligonucleotides / chemistry
Plasmids / metabolism
Protein Binding
Protein Conformation
Protein Structure, Secondary
Protein Structure, Tertiary
Sequence Homology, Amino Acid
Telomere / chemistry
Telomere / ultrastructure
|
| IF |
4.238
|
| 引用数 |
18
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| カイコ |
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