RRC ID |
33607
|
著者 |
Fleckenstein T, Kastenmüller A, Stein ML, Peters C, Daake M, Krause M, Weinfurtner D, Haslbeck M, Weinkauf S, Groll M, Buchner J.
|
タイトル |
The Chaperone Activity of the Developmental Small Heat Shock Protein Sip1 Is Regulated by pH-Dependent Conformational Changes.
|
ジャーナル |
Mol Cell
|
Abstract |
Small heat shock proteins (sHsps) are ubiquitous molecular chaperones that prevent the aggregation of unfolding proteins during proteotoxic stress. In Caenorhabditis elegans, Sip1 is the only sHsp exclusively expressed in oocytes and embryos. Here, we demonstrate that Sip1 is essential for heat shock survival of reproducing adults and embryos. X-ray crystallography and electron microscopy revealed that Sip1 exists in a range of well-defined globular assemblies consisting of two half-spheres, each made of dimeric "spokes." Strikingly, the oligomeric distribution of Sip1 as well as its chaperone activity depend on pH, with a trend toward smaller species and higher activity at acidic conditions such as present in nematode eggs. The analysis of the interactome shows that Sip1 has a specific substrate spectrum including proteins that are essential for embryo development.
|
巻・号 |
58(6)
|
ページ |
1067-78
|
公開日 |
2015-6-18
|
DOI |
10.1016/j.molcel.2015.04.019
|
PII |
S1097-2765(15)00301-9
|
PMID |
26009280
|
MeSH |
Amino Acid Sequence
Animals
Blotting, Western
Caenorhabditis elegans / genetics
Caenorhabditis elegans / metabolism
Caenorhabditis elegans Proteins / chemistry*
Caenorhabditis elegans Proteins / classification
Caenorhabditis elegans Proteins / genetics
Caenorhabditis elegans Proteins / metabolism
Cryoelectron Microscopy
Crystallography, X-Ray
Heat-Shock Proteins, Small / chemistry*
Heat-Shock Proteins, Small / genetics
Heat-Shock Proteins, Small / metabolism
Hydrogen-Ion Concentration
Models, Molecular
Molecular Chaperones / chemistry*
Molecular Chaperones / genetics
Molecular Chaperones / metabolism
Molecular Sequence Data
Mutation
Phylogeny
Protein Binding
Protein Conformation*
Protein Multimerization
Protein Structure, Quaternary
Protein Structure, Tertiary
Sequence Homology, Amino Acid
Temperature
|
IF |
15.584
|
引用数 |
29
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
リソース情報 |
線虫 |
tm3624 (sip-1
F43D9.4) |