論文 - 詳細
| RRC ID | 33674 |
|---|---|
| 著者 | Banjo T, Grajcarek J, Yoshino D, Osada H, Miyasaka KY, Kida YS, Ueki Y, Nagayama K, Kawakami K, Matsumoto T, Sato M, Ogura T. |
| タイトル | Haemodynamically dependent valvulogenesis of zebrafish heart is mediated by flow-dependent expression of miR-21. |
| ジャーナル | Nat Commun |
| Abstract |
Heartbeat is required for normal development of the heart, and perturbation of intracardiac flow leads to morphological defects resembling congenital heart diseases. These observations implicate intracardiac haemodynamics in cardiogenesis, but the signalling cascades connecting physical forces, gene expression and morphogenesis are largely unknown. Here we use a zebrafish model to show that the microRNA, miR-21, is crucial for regulation of heart valve formation. Expression of miR-21 is rapidly switched on and off by blood flow. Vasoconstriction and increasing shear stress induce ectopic expression of miR-21 in the head vasculature and heart. Flow-dependent expression of mir-21 governs valvulogenesis by regulating the expression of the same targets as mouse/human miR-21 (sprouty, pdcd4, ptenb) and induces cell proliferation in the valve-forming endocardium at constrictions in the heart tube where shear stress is highest. We conclude that miR-21 is a central component of a flow-controlled mechanotransduction system in a physicogenetic regulatory loop. |
| 巻・号 | 4 |
| ページ | 1978 |
| 公開日 | 2013-1-1 |
| DOI | 10.1038/ncomms2978 |
| PII | ncomms2978 |
| PMID | 23748970 |
| PMC | PMC3709480 |
| MeSH | 3' Untranslated Regions / genetics Animals Base Sequence Blood Circulation / drug effects Blood Circulation / physiology* Butadienes / pharmacology Diacetyl / analogs & derivatives Diacetyl / pharmacology Gene Expression Regulation, Developmental / drug effects HEK293 Cells Heart Valves / embryology* Heart Valves / metabolism Hemodynamics* Humans MAP Kinase Signaling System / drug effects MicroRNAs / genetics* MicroRNAs / metabolism Molecular Sequence Data Morpholinos / pharmacology Nitriles / pharmacology Phenotype RNA, Messenger / genetics RNA, Messenger / metabolism Sequence Alignment Zebrafish / embryology* Zebrafish / genetics Zebrafish / physiology* Zebrafish Proteins / metabolism |
| IF | 12.121 |
| 引用数 | 46 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 8 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ゼブラフィッシュ | ko095/fli1:EGFP hspGFF3A |