論文 - 詳細
| RRC ID | 3373 |
|---|---|
| 著者 | Arkblad EL, Tuck S, Pestov NB, Dmitriev RI, Kostina MB, Stenvall J, Tranberg M, Rydström J. |
| タイトル | A Caenorhabditis elegans mutant lacking functional nicotinamide nucleotide transhydrogenase displays increased sensitivity to oxidative stress. |
| ジャーナル | Free Radic Biol Med |
| Abstract |
Proton-translocating mitochondrial nicotinamide nucleotide transhydrogenase (NNT) was investigated regarding its physiological role in Caenorhabditis elegans. NNT catalyzes the reduction of NADP(+) by NADH driven by the electrochemical proton gradient, Deltap, and is thus a potentially important source of mitochondrial NADPH. Mitochondrial detoxification of reactive oxygen species (ROS) by glutathione-dependent peroxidases depends on NADPH for regeneration of reduced glutathione. Transhydrogenase may therefore be directly involved in the defense against oxidative stress. nnt-1 deletion mutants of C. elegans, nnt-1(sv34), were isolated and shown to grow essentially as wild type under normal laboratory conditions, but with a strongly lowered GSH/GSSG ratio. Under conditions of oxidative stress, caused by the superoxide-generating agent methyl viologen, growth of worms lacking nnt-1 activity was severely impaired. A similar result was obtained by using RNAi. Reintroducing nnt-1 in the nnt-1(sv34) knockout mutant led to a partial rescue of growth under oxidative stress conditions. These results provide evidence for the first time that nnt-1 is important in the defense against mitochondrial oxidative stress. |
| 巻・号 | 38(11) |
| ページ | 1518-25 |
| 公開日 | 2005-6-1 |
| DOI | 10.1016/j.freeradbiomed.2005.02.012 |
| PII | S0891-5849(05)00084-5 |
| PMID | 15890626 |
| MeSH | Animals Caenorhabditis elegans / genetics* Caenorhabditis elegans Proteins / physiology Cell Proliferation Electrochemistry Gene Deletion Glutathione Green Fluorescent Proteins / metabolism Immunoblotting Mitochondria / metabolism Models, Chemical Models, Genetic Mutation* NADP / chemistry NADP Transhydrogenases / genetics* NADP Transhydrogenases / physiology Oxidative Stress Paraquat / pharmacology Phenotype Plasmids / metabolism Protons RNA Interference RNA, Double-Stranded / chemistry Time Factors |
| IF | 6.17 |
| 引用数 | 77 |
| WOS 分野 | ENDOCRINOLOGY & METABOLISM BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm358 |