RRC ID 3397
Author Rhiner C, Gysi S, Fröhli E, Hengartner MO, Hajnal A.
Title Syndecan regulates cell migration and axon guidance in C. elegans.
Journal Development
Abstract During nervous system development, axons that grow out simultaneously in the same extracellular environment are often sorted to different target destinations. As there is only a restricted set of guidance cues known, regulatory mechanisms are likely to play a crucial role in controlling cell migration and axonal pathfinding. Heparan sulfate proteoglycans (HSPGs) carry long chains of differentially modified sugar residues that have been proposed to encode specific information for nervous system development. Here, we show that the cell surface proteoglycan syndecan SDN-1 functions autonomously in neurons to control the neural migration and guidance choices of outgrowing axons. Epistasis analysis suggests that heparan sulfate (HS) attached to SDN-1 can regulate guidance signaling by the Slit/Robo pathway. Furthermore, SDN-1 acts in parallel with other HSPG core proteins whose HS side chains are modified by the C5-epimerase HSE-5, and/or the 2O-sulfotransferase HST-2, depending on the cellular context. Taken together, our experiments show that distinct HS modification patterns on SDN-1 are involved in regulating axon guidance and cell migration in C. elegans.
Volume 132(20)
Pages 4621-33
Published 2005-10
DOI 10.1242/dev.02042
PII dev.02042
PMID 16176946
MeSH Alleles Animals Axons / physiology* Body Patterning Caenorhabditis elegans / cytology* Caenorhabditis elegans / genetics Caenorhabditis elegans / growth & development Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / metabolism Carbohydrate Epimerases / metabolism Cell Movement* Gene Expression Regulation, Developmental Genotype Membrane Glycoproteins / deficiency Membrane Glycoproteins / genetics Membrane Glycoproteins / metabolism* Mutation / genetics Nerve Tissue Proteins / metabolism Phenotype Proteoglycans / deficiency Proteoglycans / genetics Proteoglycans / metabolism* Receptors, Immunologic / metabolism Signal Transduction Syndecans
IF 5.763
Times Cited 72
WOS Category DEVELOPMENTAL BIOLOGY
Resource
C.elegans tm472