論文 - 詳細
| RRC ID | 33971 |
|---|---|
| 著者 | Fujikawa Y, Morisaki F, Ogura A, Morohashi K, Enya S, Niwa R, Goto S, Kojima H, Okabe T, Nagano T, Inoue H. |
| タイトル | A practical fluorogenic substrate for high-throughput screening of glutathione S-transferase inhibitors. |
| ジャーナル | Chem Commun (Camb) |
| Abstract |
We report a new fluorogenic substrate for glutathione S-transferase (GST), 3,4-DNADCF, enabling the assay with a low level of nonenzymatic background reaction. Inhibitors against Noppera-bo/GSTe14 from Drosophila melanogaster were identified by high throughput screening using 3,4-DNADCF, demonstrating the utility of this substrate. |
| 巻・号 | 51(57) |
| ページ | 11459-62 |
| 公開日 | 2015-7-21 |
| DOI | 10.1039/c5cc02067k |
| PMID | 26090533 |
| MeSH | Animals Drosophila Proteins / antagonists & inhibitors* Drosophila Proteins / metabolism Drosophila melanogaster / enzymology* Drug Evaluation, Preclinical Enzyme Assays Enzyme Inhibitors / pharmacology* Fluorescent Dyes / chemistry Fluorescent Dyes / metabolism* Glutathione / chemistry Glutathione / metabolism Glutathione Transferase / antagonists & inhibitors* Glutathione Transferase / metabolism High-Throughput Screening Assays Humans Spectrometry, Fluorescence Substrate Specificity |
| IF | 5.996 |
| 引用数 | 12 |
| WOS 分野 | CHEMISTRY, MULTIDISCIPLINARY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | Genome Network human cDNA clone IRAL015K15 (HGY086255) IRAL037F09 (HGY094929) IRAK022O02 (HGX009138) IRAL007N16 (HGY083128) IRAL016K22 (HGY086662) IRAL005A23 (HGY082023) IRAK004O20 (HGX001956) IRAL006E09 (HGY082505) IRAL004F23 (HGY081743) |