論文 - 詳細
| RRC ID | 34010 |
|---|---|
| 著者 | Satomi M, Furushita M, Oikawa H, Yoshikawa-Takahashi M, Yano Y. |
| タイトル | Analysis of a 30 kbp plasmid encoding histidine decarboxylase gene in Tetragenococcus halophilus isolated from fish sauce. |
| ジャーナル | Int J Food Microbiol |
| Abstract |
In order to analyze the genes related to the histamine production, a strain of histamine producing halophilic bacteria, referred to as strain H, was isolated using enrichment culture and dilution-to-extinction methods with histidine broth inoculated from the fish sauce mashes. The two Japanese fish sauce mashes used, accumulate over 1000 mg/l of histamine. Phenotypic and 16 S rRNA gene sequence analyses identified strain H as Tetragenococcus halophilus, the predominant histamine producing bacteria present during fish sauce fermentation. Genetic analyses (PCR and Southern blot) of the histamine producing strain confirmed that the strain harbored a 30 kbp plasmid (pHDC) encoding a single copy of the pyruvoyl dependent histidine decarboxylase gene (hdc). A comparison of hdcA that is a structural gene of histidine decarboxylase among strain H, Lactobacillus hilgardii 0006, L. sakei LTH2076, Oenococcus oeni 9204, T. halophilus and T. muriaticus JCM10006 (T) indicated >99% sequence similarity. The hdc gene cluster consisted of 4 ORFs, hdcP, hdcA, hdcB, and hdcRS, and were almost identical to that of L. hilgardii 0006 with 99% sequence similarity including the structural hdc spacer region. However, the approximately 500 bp regions upstream and downstream of the hdc gene were different between that of strain H and L. hilgardii 0006. The complete sequence of pHDC revealed 29,924 nucleotides including 28 ORFs, two pairs of IR (inverted repeat), similar sequence of plasmid conjugative elements, and a theta-type replicon. These results suggested that hdc could be encoded on transformable elements among lactic acid bacteria. |
| 巻・号 | 126(1-2) |
| ページ | 202-9 |
| 公開日 | 2008-8-15 |
| DOI | 10.1016/j.ijfoodmicro.2008.05.025 |
| PII | S0168-1605(08)00298-5 |
| PMID | 18573560 |
| MeSH | Base Sequence Cloning, Molecular Cluster Analysis Fermentation Fish Products / microbiology* Food Microbiology Foodborne Diseases / microbiology* Histamine / biosynthesis* Histidine Decarboxylase / genetics* Histidine Decarboxylase / metabolism Lactobacillus / classification Lactobacillus / enzymology* Molecular Sequence Data Molecular Weight Phylogeny Plasmids / genetics* Polymerase Chain Reaction Sequence Analysis, DNA |
| IF | 4.187 |
| 引用数 | 56 |
| WOS 分野 | FOOD SCIENCE & TECHNOLOGY MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 19268 JCM 19269 |