RRC ID 34129
Author Tamai R, Kiyoura Y.
Title Candida albicans and Candida parapsilosis rapidly up-regulate galectin-3 secretion by human gingival epithelial cells.
Journal Mycopathologia
Abstract Galectin-3 is a β-galactoside-binding C-type lectin that plays an important role in innate immunity. The purpose of this study was to determine whether Candida albicans and Candida parapsilosis up-regulate galectin-3 secretion by human gingival epithelial cells and gingival fibroblasts. Ca9-22, a human gingival epithelial cell line, and human gingival fibroblasts were incubated in the presence or absence of C. albicans or C. parapsilosis without serum. Levels of secreted human galectin-3 in culture supernatants were measured by enzyme-linked immunosorbent assay. We also pretreated Ca9-22 cells with cytochalasin D (an actin polymerization inhibitor), ALLN (a calpain inhibitor) and LY294002 [a phosphatidylinositol-3 kinase (PI3K) inhibitor] to determine whether the up-regulation of galectin-3 secretion was mediated by cytoskeletal changes, protease activity, or PI3K signaling. Galectin-3 secretion was significantly and rapidly up-regulated by live C. albicans and C. parapsilosis, as well as heat-killed C. albicans. In addition, cytochalasin D, LY294002 and ALLN did not inhibit the up-regulation in galectin-3 secretion. These results suggest that both live and heat-killed C. albicans and C. parapsilosis may increase the activity of the innate immune system and invasion by other microorganisms via up-regulation of galectin-3 secretion.
Volume 177(1-2)
Pages 75-9
Published 2014-2
DOI 10.1007/s11046-013-9725-1
PMID 24436012
MeSH Actins / antagonists & inhibitors Calpain / antagonists & inhibitors Candida albicans / immunology* Cell Line Chromones / pharmacology Cytochalasin D / pharmacology Enzyme-Linked Immunosorbent Assay Epithelial Cells / metabolism* Fibroblasts Galectin 3 / biosynthesis Galectin 3 / metabolism* Gingiva / cytology Gingiva / immunology* Gingiva / microbiology Humans Leupeptins / pharmacology Morpholines / pharmacology Phosphoinositide-3 Kinase Inhibitors
IF 2.278
Times Cited 6
General Microbes JCM 1612