論文 - 詳細
| RRC ID | 34129 |
|---|---|
| 著者 | Tamai R, Kiyoura Y. |
| タイトル | Candida albicans and Candida parapsilosis rapidly up-regulate galectin-3 secretion by human gingival epithelial cells. |
| ジャーナル | Mycopathologia |
| Abstract |
Galectin-3 is a β-galactoside-binding C-type lectin that plays an important role in innate immunity. The purpose of this study was to determine whether Candida albicans and Candida parapsilosis up-regulate galectin-3 secretion by human gingival epithelial cells and gingival fibroblasts. Ca9-22, a human gingival epithelial cell line, and human gingival fibroblasts were incubated in the presence or absence of C. albicans or C. parapsilosis without serum. Levels of secreted human galectin-3 in culture supernatants were measured by enzyme-linked immunosorbent assay. We also pretreated Ca9-22 cells with cytochalasin D (an actin polymerization inhibitor), ALLN (a calpain inhibitor) and LY294002 [a phosphatidylinositol-3 kinase (PI3K) inhibitor] to determine whether the up-regulation of galectin-3 secretion was mediated by cytoskeletal changes, protease activity, or PI3K signaling. Galectin-3 secretion was significantly and rapidly up-regulated by live C. albicans and C. parapsilosis, as well as heat-killed C. albicans. In addition, cytochalasin D, LY294002 and ALLN did not inhibit the up-regulation in galectin-3 secretion. These results suggest that both live and heat-killed C. albicans and C. parapsilosis may increase the activity of the innate immune system and invasion by other microorganisms via up-regulation of galectin-3 secretion. |
| 巻・号 | 177(1-2) |
| ページ | 75-9 |
| 公開日 | 2014-2-1 |
| DOI | 10.1007/s11046-013-9725-1 |
| PMID | 24436012 |
| MeSH | Actins / antagonists & inhibitors Blood Proteins Calpain / antagonists & inhibitors Candida albicans / immunology* Cell Line Chromones / pharmacology Cytochalasin D / pharmacology Enzyme-Linked Immunosorbent Assay Epithelial Cells / metabolism* Fibroblasts Galectin 3 / biosynthesis Galectin 3 / metabolism* Galectins Gingiva / cytology Gingiva / immunology* Gingiva / microbiology Humans Leupeptins / pharmacology Morpholines / pharmacology Phosphoinositide-3 Kinase Inhibitors |
| IF | 2.452 |
| 引用数 | 10 |
| WOS 分野 | MYCOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 1612 |