論文 - 詳細
| RRC ID | 35273 |
|---|---|
| 著者 | Levin-Reisman I, Gefen O, Fridman O, Ronin I, Shwa D, Sheftel H, Balaban NQ. |
| タイトル | Automated imaging with ScanLag reveals previously undetectable bacterial growth phenotypes. |
| ジャーナル | Nat Methods |
| Abstract |
We developed an automated system, ScanLag, that measures in parallel the delay in growth (lag time) and growth rate of thousands of cells. Using ScanLag, we detected small subpopulations of bacteria with dramatically increased lag time upon starvation. By screening a library of Escherichia coli deletion mutants, we achieved two-dimensional mapping of growth characteristics, which showed that ScanLag enables multidimensional screens for quantitative characterization and identification of rare phenotypic variants. |
| 巻・号 | 7(9) |
| ページ | 737-9 |
| 公開日 | 2010-9-1 |
| DOI | 10.1038/nmeth.1485 |
| PII | nmeth.1485 |
| PMID | 20676109 |
| MeSH | Automation Escherichia coli / genetics Escherichia coli / growth & development* Gene Library Genetic Variation Image Processing, Computer-Assisted / methods* Mutation Phenotype |
| IF | 30.822 |
| 引用数 | 67 |
| WOS 分野 | BIOCHEMICAL RESEARCH METHODS |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 30 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | Keio collection the large and medium deletion mutants library |