論文 - 詳細
| RRC ID | 3564 |
|---|---|
| 著者 | Choe KP, Strange K. |
| タイトル | Evolutionarily conserved WNK and Ste20 kinases are essential for acute volume recovery and survival after hypertonic shrinkage in Caenorhabditis elegans. |
| ジャーナル | Am J Physiol Cell Physiol |
| Abstract |
Members of the germinal center kinase (GCK)-VI subfamily of Ste20 kinases regulate a Caenorhabditis elegans ClC anion channel and vertebrate SLC12 cation-Cl(-) cotransporters. With no lysine (K) (WNK) protein kinases interact with and activate the mammalian GCK-VI kinases proline-alanine-rich Ste20-related kinase (PASK) and oxidative stress-responsive 1 (OSR1). We demonstrate here for the first time that GCK-VI kinases play an essential role in whole animal osmoregulation. RNA interference (RNAi) knockdown of the single C. elegans GCK-VI kinase, GCK-3, dramatically inhibits systemic volume recovery and survival after hypertonic shrinkage. Tissue-specific RNAi suggests that GCK-3 functions primarily in the hypodermis and intestine to mediate volume recovery. The single C. elegans WNK kinase, WNK-1, binds to GCK-3, and wnk-1 knockdown gives rise to a phenotype qualitatively similar to that of gck-3(RNAi) worms. Knockdown of the two kinases together has no additive effect, suggesting that WNK-1 and GCK-3 function in a common pathway. We postulate that WNK-1 functions upstream of GCK-3 in a manner similar to that postulated for its mammalian homologs. Phylogenetic analysis of kinase functional domains suggests that the interaction between GCK-VI and WNK kinases first occurred in an early metazoan and therefore likely coincided with the need of multicellular animals to tightly regulate transepithelial transport processes that mediate systemic osmotic homeostasis. |
| 巻・号 | 293(3) |
| ページ | C915-27 |
| 公開日 | 2007-9-1 |
| DOI | 10.1152/ajpcell.00126.2007 |
| PII | 00126.2007 |
| PMID | 17596296 |
| MeSH | Amino Acid Sequence Animals Animals, Genetically Modified Caenorhabditis elegans / enzymology Caenorhabditis elegans / genetics* Caenorhabditis elegans Proteins / genetics* Caenorhabditis elegans Proteins / metabolism Evolution, Molecular Germinal Center Kinases Green Fluorescent Proteins / genetics Intestines / enzymology Molecular Sequence Data Osmotic Pressure Phylogeny Protein Kinases / genetics* Protein Kinases / metabolism Protein Serine-Threonine Kinases / genetics* Protein Serine-Threonine Kinases / metabolism RNA Interference Saline Solution, Hypertonic / pharmacology Subcutaneous Tissue / enzymology WNK Lysine-Deficient Protein Kinase 1 Water-Electrolyte Balance / physiology* |
| IF | 3.485 |
| 引用数 | 55 |
| WOS 分野 | PHYSIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | F1000Research |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm1223 |