論文 - 詳細
| RRC ID | 35715 |
|---|---|
| 著者 | Schoborg T, Zajac AL, Fagerstrom CJ, Guillen RX, Rusan NM. |
| タイトル | An Asp-CaM complex is required for centrosome-pole cohesion and centrosome inheritance in neural stem cells. |
| ジャーナル | J Cell Biol |
| Abstract |
The interaction between centrosomes and mitotic spindle poles is important for efficient spindle formation, orientation, and cell polarity. However, our understanding of the dynamics of this relationship and implications for tissue homeostasis remains poorly understood. Here we report that Drosophila melanogaster calmodulin (CaM) regulates the ability of the microcephaly-associated protein, abnormal spindle (Asp), to cross-link spindle microtubules. Both proteins colocalize on spindles and move toward spindle poles, suggesting that they form a complex. Our binding and structure-function analysis support this hypothesis. Disruption of the Asp-CaM interaction alone leads to unfocused spindle poles and centrosome detachment. This behavior leads to randomly inherited centrosomes after neuroblast division. We further show that spindle polarity is maintained in neuroblasts despite centrosome detachment, with the poles remaining stably associated with the cell cortex. Finally, we provide evidence that CaM is required for Asp's spindle function; however, it is completely dispensable for Asp's role in microcephaly suppression. |
| 巻・号 | 211(5) |
| ページ | 987-98 |
| 公開日 | 2015-12-7 |
| DOI | 10.1083/jcb.201509054 |
| PII | jcb.201509054 |
| PMID | 26620907 |
| PMC | PMC4674283 |
| MeSH | Animals Brain / pathology Calmodulin / metabolism* Cell Division Cell Line Cell Polarity Centrosome / metabolism* Clustered Regularly Interspaced Short Palindromic Repeats Drosophila Proteins / metabolism* Drosophila melanogaster / metabolism Exons Genotype Green Fluorescent Proteins / metabolism Microtubule-Associated Proteins / metabolism* Microtubules / metabolism Mutation Neural Stem Cells / cytology* Phenotype Protein Binding RNA Interference Spindle Apparatus / metabolism |
| IF | 8.811 |
| 引用数 | 19 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 5 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ショウジョウバエ | CAS-0004 |