RRC ID 35715
Author Schoborg T, Zajac AL, Fagerstrom CJ, Guillen RX, Rusan NM.
Title An Asp-CaM complex is required for centrosome-pole cohesion and centrosome inheritance in neural stem cells.
Journal J Cell Biol
Abstract The interaction between centrosomes and mitotic spindle poles is important for efficient spindle formation, orientation, and cell polarity. However, our understanding of the dynamics of this relationship and implications for tissue homeostasis remains poorly understood. Here we report that Drosophila melanogaster calmodulin (CaM) regulates the ability of the microcephaly-associated protein, abnormal spindle (Asp), to cross-link spindle microtubules. Both proteins colocalize on spindles and move toward spindle poles, suggesting that they form a complex. Our binding and structure-function analysis support this hypothesis. Disruption of the Asp-CaM interaction alone leads to unfocused spindle poles and centrosome detachment. This behavior leads to randomly inherited centrosomes after neuroblast division. We further show that spindle polarity is maintained in neuroblasts despite centrosome detachment, with the poles remaining stably associated with the cell cortex. Finally, we provide evidence that CaM is required for Asp's spindle function; however, it is completely dispensable for Asp's role in microcephaly suppression.
Volume 211(5)
Pages 987-98
Published 2015-12-7
DOI 10.1083/jcb.201509054
PII jcb.201509054
PMID 26620907
PMC PMC4674283
MeSH Animals Brain / pathology Calmodulin / metabolism* Cell Division Cell Line Cell Polarity Centrosome / metabolism* Clustered Regularly Interspaced Short Palindromic Repeats Drosophila Proteins / metabolism* Drosophila melanogaster / metabolism Exons Genotype Green Fluorescent Proteins / metabolism Microtubule-Associated Proteins / metabolism* Microtubules / metabolism Mutation Neural Stem Cells / cytology* Phenotype Protein Binding RNA Interference Spindle Apparatus / metabolism
IF 8.811
Times Cited 19
Drosophila CAS-0004