論文 - 詳細
| RRC ID | 35735 |
|---|---|
| 著者 | Pichugina T, Sugawara T, Kaykov A, Schierding W, Masuda K, Uewaki J, Grand RS, Allison JR, Martienssen RA, Nurse P, Ueno M, O'Sullivan JM. |
| タイトル | A diffusion model for the coordination of DNA replication in Schizosaccharomyces pombe. |
| ジャーナル | Sci Rep |
| Abstract |
The locations of proteins and epigenetic marks on the chromosomal DNA sequence are believed to demarcate the eukaryotic genome into distinct structural and functional domains that contribute to gene regulation and genome organization. However, how these proteins and epigenetic marks are organized in three dimensions remains unknown. Recent advances in proximity-ligation methodologies and high resolution microscopy have begun to expand our understanding of these spatial relationships. Here we use polymer models to examine the spatial organization of epigenetic marks, euchromatin and heterochromatin, and origins of replication within the Schizosaccharomyces pombe genome. These models incorporate data from microscopy and proximity-ligation experiments that inform on the positions of certain elements and contacts within and between chromosomes. Our results show a striking degree of compartmentalization of epigenetic and genomic features and lead to the proposal of a diffusion based mechanism, centred on the spindle pole body, for the coordination of DNA replication in S. pombe. |
| 巻・号 | 6 |
| ページ | 18757 |
| 公開日 | 2016-1-5 |
| DOI | 10.1038/srep18757 |
| PII | srep18757 |
| PMID | 26729303 |
| PMC | PMC4700429 |
| MeSH | Cell Cycle Proteins / genetics Cell Cycle Proteins / metabolism Cell Nucleus / genetics DNA Replication* DNA, Fungal* Euchromatin / genetics G1 Phase / genetics Genome, Fungal Genomics Heterochromatin / genetics Histone-Lysine N-Methyltransferase Methyltransferases / genetics Methyltransferases / metabolism Models, Biological* Replication Origin Schizosaccharomyces / genetics* Schizosaccharomyces / metabolism Schizosaccharomyces pombe Proteins / genetics Schizosaccharomyces pombe Proteins / metabolism Sequence Deletion |
| IF | 3.998 |
| 引用数 | 9 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 15 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 酵母 | FY17781 FY17780 FY17791 FY15592 FY14816 FY15591 FY15550 |