RRC ID 35735
Author Pichugina T, Sugawara T, Kaykov A, Schierding W, Masuda K, Uewaki J, Grand RS, Allison JR, Martienssen RA, Nurse P, Ueno M, O'Sullivan JM.
Title A diffusion model for the coordination of DNA replication in Schizosaccharomyces pombe.
Journal Sci Rep
Abstract The locations of proteins and epigenetic marks on the chromosomal DNA sequence are believed to demarcate the eukaryotic genome into distinct structural and functional domains that contribute to gene regulation and genome organization. However, how these proteins and epigenetic marks are organized in three dimensions remains unknown. Recent advances in proximity-ligation methodologies and high resolution microscopy have begun to expand our understanding of these spatial relationships. Here we use polymer models to examine the spatial organization of epigenetic marks, euchromatin and heterochromatin, and origins of replication within the Schizosaccharomyces pombe genome. These models incorporate data from microscopy and proximity-ligation experiments that inform on the positions of certain elements and contacts within and between chromosomes. Our results show a striking degree of compartmentalization of epigenetic and genomic features and lead to the proposal of a diffusion based mechanism, centred on the spindle pole body, for the coordination of DNA replication in S. pombe.
Volume 6
Pages 18757
Published 2016-1-5
DOI 10.1038/srep18757
PII srep18757
PMID 26729303
PMC PMC4700429
MeSH Cell Cycle Proteins / genetics Cell Cycle Proteins / metabolism Cell Nucleus / genetics DNA Replication* DNA, Fungal* Euchromatin / genetics G1 Phase / genetics Genome, Fungal Genomics Heterochromatin / genetics Methyltransferases / genetics Methyltransferases / metabolism Models, Biological* Replication Origin Schizosaccharomyces / genetics* Schizosaccharomyces / metabolism Schizosaccharomyces pombe Proteins / genetics Schizosaccharomyces pombe Proteins / metabolism Sequence Deletion
IF 4.011
Times Cited 6
Yeast FY17781 FY17780 FY17791 FY15592 FY14816 FY15591 FY15550