RRC ID 3576
著者 Grill B, Bienvenut WV, Brown HM, Ackley BD, Quadroni M, Jin Y.
タイトル C. elegans RPM-1 regulates axon termination and synaptogenesis through the Rab GEF GLO-4 and the Rab GTPase GLO-1.
ジャーナル Neuron
Abstract C. elegans RPM-1 (for Regulator of Presynaptic Morphology) is a member of a conserved protein family that includes Drosophila Highwire and mammalian Pam and Phr1. These are large proteins recently shown to regulate synaptogenesis through E3 ubiquitin ligase activities. Here, we report the identification of an RCC1-like guanine nucleotide exchange factor, GLO-4, from mass spectrometry analysis of RPM-1-associated proteins. GLO-4 colocalizes with RPM-1 at presynaptic terminals. Loss of function in glo-4 or in its target Rab GTPase, glo-1, causes neuronal defects resembling those in rpm-1 mutants. We show that the glo pathway functions downstream of rpm-1 and acts in parallel to fsn-1, a partner of RPM-1 E3 ligase function. We find that late endosomes are specifically disorganized at the presynaptic terminals of glo-4 mutants. Our data suggest that RPM-1 positively regulates a Rab GTPase pathway to promote vesicular trafficking via late endosomes.
巻・号 55(4)
ページ 587-601
公開日 2007-8-16
DOI 10.1016/j.neuron.2007.07.009
PII S0896-6273(07)00530-2
PMID 17698012
MeSH Animals Animals, Genetically Modified Caenorhabditis elegans Caenorhabditis elegans Proteins / analysis Caenorhabditis elegans Proteins / metabolism* Caenorhabditis elegans Proteins / physiology* Green Fluorescent Proteins / metabolism Guanine Nucleotide Exchange Factors / analysis Guanine Nucleotide Exchange Factors / metabolism* Guanine Nucleotide Exchange Factors / physiology* Immunoprecipitation / methods Mass Spectrometry / methods Mutation / physiology Neurons / classification Neurons / cytology* Neurons / metabolism Presynaptic Terminals / physiology* Protein Transport / physiology Subcellular Fractions / metabolism Synapses / physiology* rab GTP-Binding Proteins / metabolism*
IF 14.415
引用数 87
WOS 分野 NEUROSCIENCES
リソース情報
線虫 tm920